大黄素干预高糖培养大鼠肾小球系膜细胞的凋亡

背景:前期工作已经证实中药复方消可宁(制大黄、制附子、生黄芪)能有效防治早期糖尿病肾病并获得国家专利(专利号:200410064899X)。目的:观察大黄主要活性成分大黄素对高糖培养的SD大鼠肾小球系膜细胞凋亡的影响。方法:用20,40,80μmol/L大黄素刺激高糖培养的SD大鼠肾小球系膜细胞,苏木精-伊红染色观察凋亡细胞形态,4’,6-二脒基-2-苯基吲哚荧光染色观察细胞核凋亡情况,流式细胞仪观察细胞凋亡率。结果与结论:苏木精-伊红染色及4’,6-二脒基-2-苯基吲哚染色结果显示大黄素对高糖培养的肾小球系膜细胞的凋亡有影响,且与浓度与时间成正相关,细胞凋亡率组间差异有显著性意义(P<0.05)。提示大黄素可诱导肾小球系膜细胞凋亡,且与药物浓度及时间成正相关。

Effect of Archen on apoptosis of rat glomerular mesangial cells cultured with high glucose

BACKGROUND:Our previous study has already manifested that Chinese medicine Xiaokening (prepared rhubarb,radix aconiti lateralis preparata and astragalus) can effectively control the development of early diabetic nephropathy and gained a national patent (patent No.200410064899X) OBJECTIVE:To investigate the effect of Archen on the apoptosis of rat glomerular mesangial cells cultured with high glucose.METHODS:Glomerular mesangial cells were stimulated by different concentrations of Archen (20,40 and 80 μmol/L).The morphology of apoptotic cells was observed by hematoxylin-eosin staining.Karyon apoptosis was examined by fluorescence staining of 4’,6-diamidino-2-phenylindole.Cell apoptosis rate was detected by flow cytometry.RESULTS AND CONCLUSION:The outcome of hematoxylin-eosin staining and 4’,6-diamidino-2-phenylindole fluorescence staining indicated that Archen could obviously induce the apoptosis of glomerular mesangial cells in a doseand time-dependent manner.There was significant difference of the apoptosis rate between groups (P < 0.05).This result shows that Archen can obviously induce apoptosis of glomerular mesangial cells in dose-and time-dependent manner.