三种贵金属烤瓷合金与高糖对小鼠成纤维细胞L-929增殖的影响

背景：糖尿病患者这一特殊群体口腔环境较为特殊,关于口腔修复材料在这类患者应用的安全性研究较少见报道。目的：检测3种贵金属烤瓷合金与高糖对体外培养的小鼠成纤维细胞L-929增殖的影响。方法：将89%金合金、74%金合金、银钯合金3种烤瓷材料浸泡在不同糖浓度（11.1,22.2,33.3mmol/L）的培养液中浸提,将所得的浸提液与小鼠成纤维细胞L-929共培养。同期设立空白对照。结果与结论：L-929在不同贵金属烤瓷合金和不同糖浓度的细胞培养液中的增殖活性不同,24,72h两因素交互作用的P值均小于0.05。与阴性对照组相比,22.2mmol/L糖浓度下89%金合金促进细胞的增殖（P=0.004）,银钯合金则抑制细胞的增殖（P〈0.001）,74%金合金在不同糖浓度的培养液中与阴性对照组相比其细胞的增殖活性差异无显著性意义。结果表明,上述3种材料中74%金合金在不同糖浓度下对细胞增殖的影响最小。

Effects of three kinds of noble ceramic alloys and high glucose on proliferation of L-929 cells in mice

BACKGROUND：The oral environments of diabetic patients were special,and there are few studies on safety of dental restorative materials for these patients.OBJECTIVE：To observe the effects of three kinds of noble ceramic alloys and high glucose on proliferation of mouse fibroblasts L-929 cultured in vitro.METHODS：89% Au-base alloy,74% Au-base alloy and silver-palladium alloy were immersed in mediums with different glucose concentrations（11.1,22.2,and 33.3 mmol/L） for extraction.Mouse fibroblasts L-929 were co-cultured with the extraction.The control group was set up.RESULTS AND CONCLUSION：Proliferative activities of L-929 cells cultured in mediums with different glucose concentrations and different noble ceramic alloys were different,and the two-factor interactions at 24 and 72 hours existed（P 0.05）.The proliferation of L-929 cells cultured by 89% Au-base alloy and 22.2 mmol/L high glucose concentration was more faster than the control group（P=0.004）,while the silver-palladium alloy could inhibit the proliferation（P 0.001）.However,no significant difference was found among the 74% Au-base alloy groups with different glucose concentrations and the control group.It is suggested that 74%Au-alloy with different glucose concentrations among these three kinds of materials has the least effects on cell proliferation.