运动条件下健康人循环内皮祖细胞的数量和功能

背景：运动条件对内皮祖细胞数量和功能是否会产生的影响目前尚无公识。目的：观察急性运动对健康成人循环内皮祖细胞数量和功能的影响。方法：健康男性成人志愿者12例参加急性平板运动锻炼（9.3±2.1）min。用流式细胞仪测定运动前后CD34和KDR双标阳性循环内皮祖细胞水平,ac-LDL及lectin荧光标记方法评估体外培养内皮祖细数量,检测内皮祖细胞的黏附、迁移和增殖能力,并测定运动前后血浆和内皮祖细胞分泌一氧化氮、血管内皮生长因子和粒-巨噬细胞集落刺激因子水平变化。结果与结论：流式细胞仪检测显示健康志愿者运动后循环内皮祖细胞水平较运动前增加（P〈0.05）。荧光标记法证实运动后ac-LDL及lectin抗体双阳性内皮祖细胞数量较运动前增加（P〈0.05）,内皮祖细胞迁移和增殖能力明显增强（P〈0.05）,但黏附能力无明显变化。急性运动能明显增加健康志愿者的血浆一氧化氮水平（P〈0.05）,健康志愿者运动前后血浆一氧化氮水平和循环内皮祖细胞数量及功能的增加倍数呈明显的直线相关回归关系（P〈0.05）。结果证实,运动可明显增加健康成人循环内皮祖细胞的数量和功能,其机制与其诱导一氧化氮释放增多有关。

Effect of exercise on number and activity of circulating endothelial progenitor cells in healthy subjects

BACKGROUND：Whether exercise can influence the number and activity of endothelial progenitor cells（EPCs） is still unclear.OBJECTIVE：To observe the effect of exercise on number and activity of circulating EPCs in healthy subjects.METHODS：Twelve male healthy volunteers underwent acute dynamic treadmill exercise with（9.3±2.1） minutes.Flow cytometry analysis was used to evaluate the number of CD34 and KDR double-positive labeled circulating EPCs before and after exercise,and acetylated-low-density lipoprotein（LDL） and lectin fluorescent staining method were used to evaluate the number of cultured EPCs.The adhesion,migration and proliferation of EPCs were detected.In addition,the nitric oxide（NO）,vascular endothelial growth factor（VEGF） and granulocyte-macrophage colony stimulating factor（GM-CSF） levels in plasma and secreted by circulating EPCs were measured before and after exercise.RESULTS AND CONCLUSION：Flow cytometry analysis showed that the number of circulating EPCs increased after exercise（P 0.05）.The acetylated-LDL and lectin fluorescent staining method indicated that the cultured EPCs also increased after exercise（P 0.05）.The proliferative and migratory activities of cultured EPCs were higher after exercise compared with before exercise（P 0.05）,but the adhesive activity did not change.Acute exercise also significantly enhanced plasma NO levels in healthy subjects（P 0.05）.There was a significant linear regression relationship between the enhanced plasma NO level and increased number or activity of circulating EPCs in healthy subjects（P 0.05）.Exercise can increase the number and activity of circulating EPCs in healthy subjects,which are related to the enhanced NO production in healthy subjects.