注射型可吸收聚氨基酸/硫酸钙复合材料动物体内降解吸收及促成骨作用

背景：注射型人工骨可经皮穿刺注射植入体内,对机体创伤较小,同时该型材料可以任意塑形,能较好地充填骨缺损,但目前临床上尚无在体内能完全降解吸收且具有较好促成骨作用的注射型人工骨产品。目的：评估注射型可吸收聚氨基酸/硫酸钙复合材料（硫酸钙含量70%）动物体内的降解吸收及促成骨作用,观察其修复骨缺损的能力。方法：取48只新西兰大白兔,在股骨外髁处制备直径为5mm、深10mm的骨缺损模型,以随机数字表法分为实验组和对照组,实验组将注射型可吸收聚氨基酸/硫酸钙复合材料植入骨缺损处,对照组未予干预。结果与结论：X射线平片示：实验组骨缺损逐渐被骨痂填充,术后16周,骨缺损处恢复正常松质骨密度,塑形完成;对照组骨缺损处修复不明显。组织学检查（苏木精-伊红、MASSON染色）示：术后4周,材料开始降解,新生原始骨小梁长入材料内;术后12周,编织骨开始转化为板层骨;术后16周,材料完全被降解吸收,新生骨组织完全修复骨缺损。结果显示注射型聚氨基酸/硫酸钙复合材料在动物内能够完全降解、吸收,具备一定的成骨活性,可望用作骨修复材料。

In vivo degradation,absorption and osteogenesis of injectable and absorbable polyamine acid/calcium sulfate composites

BACKGROUND：With fewer traumas to the body,injectable artificial bone can be implanted into the body through the percutaneous injection.Meanwhile,the material can be any shape to fill bone defects.But now in clinic,there is no injectable artificial bone substitute product with the better ability of bone formation,which can be completely degraded and absorbed in vivo.OBJECTIVE：To evaluate the degradation,absorption and osteogenesis in vivo ability of injectable and absorbable polyamine acid/calcium sulfate（PAA/CS） composites（containing 70% calcium sulfate）,and to observe the ability to repair bone defects.METHODS：A 5 mm×10 mm size bone defect model was made on the femoral condyle of 48 New Zealand white rabbits,which were randomly divided into experimental group（the defect was implanted with injectable and absorbable PAA/CS composites） and blank control group（without intervention）.RESULTS AND CONCLUSION：X-ray examination showed the defect of the experimental group was gradually filled by callus,at 16 weeks postoperation,the bone defects were recovered to normal cancellous bone density and the moulding was finished;the defects of the blank control group were inconspicuously repaired.Histological examination（hematoxylin-eosin staining and MASSON staining） showed that at 4 weeks postoperation,the material began to degrade and new original trabecular bone extended into the material;at 12 weeks postoperation,woven bone turned into shelf bone;at 16 weeks postoperation,the material was completely degraded and absorbed and the bone defects were repaired by new bone tissue completely.The injectable and absorbable PAA/CS composites can be completely degraded and absorbed in vivo,with a certain activity of bone formation,expected to be used as bone repair materials.