碱性成纤维细胞生长因子可拮抗过氧化氢对软骨细胞的损伤

背景：碱性成纤维细胞生长因子是否能够拮抗氧自由基带来的软骨细胞损害尚不清楚。目的：观察碱性成纤维细胞生长因子对过氧化氢诱导的大鼠膝关节软骨细胞损伤的拮抗作用。方法：分别以0.05,0.1,0.2,0.4,0.8,1.6,3.2mmol/L的过氧化氢诱导SD大鼠膝关节软骨细胞凋亡,从中选择有效的诱导凋亡浓度同时加入1μg/L的碱性成纤维细胞生长因子进行干预。结果与结论：MTT结果显示0.2～1.6mmol/L的过氧化氢均可明显诱导SD大鼠膝关节软骨细胞发生凋亡,且随浓度增加,凋亡增加;Hoechst33342染色及流式细胞仪检测发现1μg/L的碱性成纤维细胞生长因子可拮抗过氧化氢诱导的大鼠软骨细胞凋亡。

Basic fibroblast growth factors antagonize hydrogen peroxide damage to chondrocytes

BACKGROUND：Whether basic fibroblast growth factor（bFGF） can antagonize hydrogen peroxide damage to chondrocytes is still unclear.OBJECTIVE：To observe the antagonism effect of bFGF on the hydrogen peroxide-induced cell damage to rat knee-joint chondrocytes.METHODS：Chondrocyte apoptosis of Sprague Dawley（SD） rat knee-joint was induced by 0.05,0.1,0.2,0.4,0.8,1.6 and 3.2 mmol/L hydrogen peroxide.Effective concentration for induced apoptosis was chosen,and then 1 μg/L bFGF was added into it for intervention.RESULTS AND CONCLUSION：The MTT data indicated that 0.2-1.6 mmol/L hydrogen peroxide could significantly induce apoptosis of chondrocytes of SD rat knee-joint.Apoptosis was increasing as the concentration was increased.The Hoechst33342 staining and flow cytometry results showed that 1 μg/L bFGF could antagonise the hydrogen peroxide-induced apoptosis to rat knee-joint chondrocytes.