兔下颌骨来源骨髓间充质干细胞的体外培养及生物学特性

背景：以往研究表明骨髓间充质干细胞的获取来源主要是股骨或髂骨,但下颌骨来源骨髓间充质干细胞的研究至今少有报道。目的：观察兔下颌骨来源的骨髓间充质干细胞体外分离、培养及其生物学特性。方法：体外分离培养兔下颌骨骨髓间充质干细胞,取第2或3代检测。MTT法检测细胞增殖,克隆形成试验检测细胞的克隆形成率变化,并对其进行成骨和成脂肪诱导分化。结果与结论：MTT法检测细胞增殖,细胞生长曲线显示下颌骨骨髓间充质干细胞经历潜伏期、对数生长期和平台期。细胞克隆形成实验显示,细胞呈成纤维集落形成单位外观。茜素红、油红O染色结果显示,成骨诱导、成脂诱导后形成钙结节及脂滴。结果证实,兔下颌骨分离出的骨髓间充质干细胞有强大的自我复制及增殖能力,具有多向诱导分化的潜能。

In vitro culture and biological characteristics of rabbit mandible-derived bone marrow mesenchymal stem cells

BACKGROUND：Previous studies have demonstrated that bone marrow mesenchymal stem cells are harvested primarily from femur or ilium,but there have been few reports describing mandible-derived bone marrow mesenchymal stem cells.OBJECTIVE：To investigate in vitro isolation,culture and biological characteristics of rabbit mandible-derived bone marrow mesenchymal stem cells.METHODS：Bone marrow mesenchymal stem cells were harvested from rabbit mandible and isolated by density gradient centrifugation.Cells were adherently cultured in vitro,and passage 2 or 3 bone marrow mesenchymal stem cells were used for detection.Cell proliferation was detected by MTT assay.Fibroblast colony forming units were detected by examination of colony formation.The potential of bone marrow mesenchymal stem cells differentiation into osteoblasts and adipocytes was detected.RESULTS AND CONCLUSION：MTT assay showed that the cell growth curves of mandible-derived bone marrow mesenchymal stem cells exhibited incubation period,logarithmic phase and platform period.Colony-formation assays showed that cells exhibited the appearance of fibroblast colony forming units.After Aalizarin red and oil red O staining,there were mineralized nodules and lipid drops.These findings suggest that rabbit mandible-derived bone marrow mesenchymal stem cells show strong self-renewal and proliferative capacities and exhibit the potential of multi-directional differentiation.