神经生长因子不同给药方式对坐骨神经吻合后修复与再生的影响

背景：神经生长因子对神经损伤后的修复有促进作用,但目前对不同用药方式的优劣性尚有争议。目的：观察鞘膜内与局部应用神经生长因子对兔坐骨神经吻合后修复与再生的影响。方法：将24只新西兰大白兔坐骨神经切断后再缝合,分别向兔鞘膜内或损伤局部注射30μg神经生长因子或等量生理盐水结果与结论：坐骨神经损伤后12周,鞘膜内注射神经生长因子组损伤坐骨神经鞘膜增厚,神经纤维排列较整齐,与正常神经纤维差异不大;且其神经干传导速度、有髓神经纤维数目、髓鞘厚度也明显高于其他组（P〈0.05）,损伤局部注射神经生长因子组次之。说明神经生长因子具有促进外周神经吻合后修复与再生的功能,鞘膜内应用优于局部注射。

Effects of nerve growth factor injection via different ways on repair and regeneration of the sciatic nerve after its anastomosis

BACKGROUND：Nerve growth factor（NGF） can promote the repair of nerve after nerve injury.However the advantages and disadvantages of different treatment methods are still unclear.OBJECTIVE：To investigate the effects of intrathecal injection and local injection of NGF on repair and regeneration of rabbit sciatic nerve after its anastomosis.METHODS：Sciatic nerves from 24 New Zealand white rabbits were cut-off and then sutured.NGF at 30 μg or saline at the same dose were injected intrathecally or locally into the rabbits.RESULTS AND CONCLUSION：After 12 weeks of sciatic nerve injury,the sheath of sciatic nerve was thickened in the intrathecal injection group,nerve fibers arranged in neat rows and was similar with normal nerve fibers;the nerve conduction velocity,number of myelinated nerve fibers and myelin sheath thickness in the intrathecal injection group were obviously increased compared with the other groups（P 0.05）,the local injection group took the second place.NGF can promote the repair and regeneration of peripheral nerve after its anastomosis,and the intrathecal injection NGF is superior to local injection.