甘油常温保存肌腱的两种方法比较

背景：最大程度保留肌腱的生物活性是进行同种异体肌腱移植的条件。目的：通过比较选择出最佳的肌腱常温保存方法。方法：使用无菌操作的方法将兔肌腱随机分为4组：新鲜肌腱对照组、生理盐水组、无水甘油Ⅰ组（肌腱梯度脱水后在无水甘油中保存）、无水甘油Ⅱ组（肌腱直接在无水甘油中保存）,分别于保存的第2,4,7个月进行检测。结果与结论：苏木精-伊红染色显示：无水甘油Ⅰ组肌腱细胞完整率明显高于无水甘油Ⅱ组。电镜观察发现,无水甘油Ⅰ组大部分肌腱细胞形态正常,肌腱组织结构完整;无水甘油Ⅱ组保存4,7个月后细胞核凝固、固缩、崩解状。无水甘油Ⅰ组的超氧化物歧化酶活性也明显高于无水甘油Ⅱ组。说明肌腱经梯度脱水后在无水甘油中常温保存效果更佳。

Comparison of two methods of tendon preservation at normal temperature in glycerine

BACKGROUND：The greatest degree to obtain the biological activity of tendon is the conditions for allograft tendon transplantation.OBJECTIVE：To select the best tendon preservation method at room temperature.METHODS：The rabbit tendons were randomly divided into four groups using the aseptic technique：Control group of fresh tendon,normal saline group,anhydrous glycerol group Ⅰ（tendons were preserved in anhydrous glycerol after gradient dehydration）,anhydrous glycerol group Ⅱ（tendons were preserved in anhydrous glycerol directly）.Tendons in each group were preformed with examination at 2,4 and 7 months.RESULTS AND CONCLUSION：Hematoxylin-eosin staining showed that the cell integrity of anhydrous glycerol group Ⅰ was higher than that of anhydrous glycerol group Ⅱ.Electron microscope observation showed that the morphology of most tendon cells in anhydrous glycerol group Ⅰ was normal,the structure of tendon tissue was integrated;After the tendons were preserved in anhydrous glycerol group Ⅱ for 4 and 7 months,the nucleus were coagulation,condensation and collapse-like.The activity of superoxide dismutase in anhydrous glycerol group Ⅰ was obviously higher than that in the anhydrous glycerol group Ⅱ.It indicates that the tendon preformed with gradient dehydration can be preserved in anhydrous glycerol effectively.