脐血间充质干细胞的适宜培养条件

背景：目前有关脐血间充质干细胞培养的实验较多,主要从改善培养基成分、脐血细胞的分离方法以及首次换液时间等方面进行调整,目前尚未建立一个安全、高效的培养体系。目的：探讨脐血间充质干细胞的适宜培养条件。方法：于新疆医科大学第一附属医院产科取健康产妇的脐血,比较不同换液时间、不同培养基、不同体积分数的胎牛血清对脐血间充质干细胞原代培养效果的影响。结果与结论：首次第5～7天换液、低糖DMEM、IMDM培养基有利于脐血间充质干细胞的生长。但还不能认为含体积分数为30%胎牛血清与含体积分数为20%胎牛血清的培养基在培养脐血间充质干细胞方面有差别。

Appropriate conditions for culture of umbilical cord blood mesenchymal stem cells

BACKGROUND：There have been many experiments describing culture of umbilical cord blood mesenchymal stem cells（UCBMSCs） in terms of improvement of medium compositions,separation method of cord blood cells,and the time for changing liquid for the first time.A safe and efficient training system has not been established yet.OBJECTIVE：To explore a better method for culture of UCBMSCs in vitro with different conditions.METHODS：The umbilical cord blood of healthy parturiens was taken from the Department of Obstetrics of the First Affiliated Hospital of Xinjiang Medical University.Several factors which influence the primary culture process of UCBMSCs were analyzed include the time for changing liquid for the first time,different culture mediums and different levels of fetal bovine serum.RESULTS AND CONCLUSION：Changing liquid at 5-7 days for the first time,low glucose DMEM or IMDE medium would be helpful to the growth of UCBMSCs.30% fetal bovine serum and 20% fetal bovine serum did not produce different effects in culture of UCBMSCs.