胶原改良聚乳酸电纺丝支架用于组织工程化输尿管的体外构建

背景：聚乳酸是一种应用广泛的细胞支架材料,但其疏水性和缺乏细胞识别信号影响了在组织工程器官构建中的应用。目的：探讨Ⅰ型胶原蛋白改良聚乳酸电纺丝支架体外构建组织工程化输尿管的可行性。方法：用Ⅰ型胶原蛋白醋酸溶液冻干法处理聚乳酸电纺丝,使Ⅰ型胶原蛋白吸附于电纺丝纤维表面,制成胶原改良电纺丝支架。将分离培养的输尿管上皮细胞分别接种于改良聚乳酸电纺丝支架和未处理的聚乳酸电纺丝支架上。结果与结论：MTT检测显示输尿管上皮细胞在改良支架中生长良好,细胞整体活性在各时间点均明显优于未处理的聚乳酸电纺丝支架上的细胞。扫描电镜观察发现细胞在改良支架表面黏附良好,接种后5d,支架表面大部分已被增殖的输尿管上皮细胞覆盖。说明胶原改良聚乳酸电纺丝支架能明显提高种子细胞的黏附和增殖活性,可用于体外构建组织工程化输尿管。

A modified electrospun polylactic acid/collagen scaffold for ureteral tissue engineering reconstruction in vitro

BACKGROUND：Polylactic acid is a widely used scaffold material,but its hydrophobicity and lack of cell recognition signal limit its application.OBJECTIVE：To investigate the feasibility of constructing a tissue engineered ureter using a modified polylactic acid/typeⅠ collagen compound scaffold in vitro.METHODS：Electrospinning polylactic acid naofibrous scaffolds were dipped into a acetic acid solution of type-Ⅰ collagen to make the collagen absorbed onto the nanofiber surface.Then ureter epithelial cells were seeded separately onto the modified electrospun polylactic acid naofibrous scaffolds or control scaffolds.RESULTS AND CONCLUSION：3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay results confirmed that there was higher cellular activity in the collagen modified compound scaffolds,which was superior to cells untreated electrospinning polylactic acid scaffold at each time point.Scanning electron microscope showed that the most part of the surface of the modified scaffold was covered with ureter epithelial cells at 5 days after seeding.The modified polylactic acid/type collagen compound scaffold can obviously increase adhesion,growth and proliferation of the seeded cells,and has potential applications in ureteral tissue engineering reconstruction.