| HPLC-MS/MS法测定大鼠血浆中LH14的含量 |
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| 引用本文： | 王彬,杨雷琼,吴卓娜,顾若兰,朱晓霞,孟志云,甘慧. HPLC-MS/MS法测定大鼠血浆中LH14的含量[J]. 解放军药学学报, 2012, 28(1): 38-40,44 |
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| 作者姓名： | 王彬 杨雷琼 吴卓娜 顾若兰 朱晓霞 孟志云 甘慧 |
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| 作者单位： | 1. 100853北京,解放军总医院检验科;100850北京,军事医学科学院野战输血研究所
2. 军事医学科学院野战输血研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金资助项目 No.NSFC20903118 |
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| 摘 要： | 目的建立SD大鼠血浆中LH14的测定方法。方法以CS55为内标,采用HPLC-MS/MS法测定,以Ulti-mate C18色谱柱(50 mm×2.1 mm,3μm)为分析柱,甲醇-水为流动相,梯度洗脱,流速为0.2 ml/min,柱温为25℃,进样量5μl。结果在2～500 ng/ml浓度范围内具有良好的线性关系,线性方程为:Y=0.0333+0.0043X,R2=0.9928(n=8)。精密度与准确度均符合生物样品的测定要求,最低定量浓度为2 ng/ml。结论该检测方法简便、准确、专属性强,能够满足LH14在SD大鼠体内药代动力学研究的需要。
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| 关 键 词： | LH14 HPLC-MS/MS 药代动力学 |
Assay for LH14 by HPLC-MS/MS in Rat Plasma |
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| WANG Bin , YANG Lei-qiong , WU Zhuo-na , GU Ruo-lan , ZHU Xiao-xia , MENG Zhi-yun , GAN Hui. Assay for LH14 by HPLC-MS/MS in Rat Plasma[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2012, 28(1): 38-40,44 |
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| Authors: | WANG Bin YANG Lei-qiong WU Zhuo-na GU Ruo-lan ZHU Xiao-xia MENG Zhi-yun GAN Hui |
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| Affiliation: | ② ① Clinical Laboratory,General Hospital of PLA,Beijing 100853,China;②Institute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing 100850,China |
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| Abstract: | Objective To develop a method for the determination of LH14 in plasma of SD rats.Methods CS55 was used as the internal standard.LH14 was chromatographed on Thermo-Finnigan TSQ Quantum with an Ultimate columnC18（50 mm×2.1 mm,3 μm）.The mobile phase was methanol-water at a flow rate of 0.2 ml/min.The column temperature was 25℃ and the injection volume was 5 μl.Results A good linear relationship was obtained between the peak area ratio of LH14/CS55 and the concentration of LH14 over the range of 2-500 ng/ml.The linear equation was Y=0.0333＋0.0043X,R2=0.9928（n=8）.The limit of quantitation was 2 ng/ml.Conclusion This method is simple,quick,accurate and suitable for the pharmacokinetic study of SD rats. |
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| Keywords: | LH14 HPLC-MS/MS pharmacokinetic |
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