血清乙肝病毒X基因的检测方法及意义

目的 探讨血清乙肝病毒（ＨＢＶ）Ｘ基因的检测方法及肝病患者Ｘ基因检测的意义。方法 通过ＰＣＲ扩增法探讨ＨＢＶ Ｘ基因的检测方法，对扩增产物的序列进行测序分析，并对３０例肝癌及３２例肝硬化患者血清ＨＢｓＡｇ、ＨＢｅＡｇ与Ｘ基因进行检测。结果经琼脂糖电泳与序列分析表明，扩增产物与Ｘ基因序列一致。血清学检测显示，２例肝癌与２例肝硬化患者血清中Ｘ基因检测阳性，但ＨＢｓＡｇ检测阴性。结论 应用ＰＣＲ法能在血清中扩增出完整Ｘ基因片段，为研究Ｘ基因的序列变化与肝癌发生的关系提供了一个有效途径。部分肝病患者血清ＨＢｓＡｇ阴性，但在血清中能检出Ｘ基因，提示肝组织中存在Ｘ基因整合。

Detective Method and Significance of Hepatitis B Virus X Gene in Serum

Objective To investigate the detective method of hepatitis B virus(HBV) X gene in serum and to study the significance of X gene detection in liver diseases. Methods HBV X gene detective method was investigated by PCR and the PCR product analysis was made by sequencing. The serum {HBsAg},HBeAg and X gene were examined in 30 patients with hepatocellular carcinoma and 32 patients with liver cirrhosis. Results The X gene was detected in nine cancer patients and fourteen liver cirrhosis patients. Two cases of hepatocellular carcinoma and two cases of liver cirrhosis were found to be HBsAg negative but X gene positive. Conclusion Serum X gene can be detected by PCR and that may play an important role in studying the relationship between X gene change and carcinogenesis. Some liver diseases with HBsAg negative in serum may be positive with X gene because of HBV X integration in liver cells.;