胃癌组织中PTEN,MMP-9和Caspase-3表达的关系

目的:研究PTEN,MMP-9和Caspase-3在胃癌及正常胃组织中的表达,探讨他们在胃癌的发生、发展、浸润和转移中的作用．方法:选择临床病理资料齐全的胃癌蜡块标本54例,另取正常胃黏膜标本15例作对照．采用SP免疫组化方法检测PTEN,MMP-9和 Caspase-3在其中的表达．结果:胃癌中PTEN低表达(28/54,51．9％),且肿瘤浸润深(P=0．004)、有淋巴(P=0．003) 和远隔转移(P=0．01 5)、临床分期高(P= 0．001)、病理分化低(P=0．008)时降低．胃癌中MMP-9高表达(41／54,75．9％),且肿瘤浸润深(P=0．040)、有淋巴转移(P=0．025)、临床分期高(P=0．039)、病理分化低(P=0．009)时增高．胃癌中Caspase-3低表达(12／54,22．2％), 且有淋巴转移(P=0．045)、临床分期高(P= 0．015)、病理分化低(P=0．035)时降低．胃癌中PTEN与MMP-9(r=-0．543,P=0．001), Caspase-3与MMP-9的表达负相关(r=0．741, P=0.001),PTEN与Caspase-3的表达正相关(r =0．515,P=0．001)．结论:胃癌中PTEN,Caspase-3低表达,MMP-9 高表达;PTEN、MMP-9和Caspase-3可作为胃癌诊断和预后判断的指标．

Relationship among PTEN,matrix metalloproteinase-9,and Caspase-3 Expression in gastric carcinoma

AIM: To study the expression of PTEN, matrix metalloproteinase-9 (MMP-9), and Caspase-3 in gastric cancer, and to discuss their roles and correlations in the occurrence, development, invasion and metastasis of gastric cancer. METHODS: SP immunohistochemistry was used to measure the expression of PTEN, MMP-9, and Caspase-3 in 54 samples of gastric carcinoma selected from paraffin wax embodied specimens with complete clinicopathological data, and another 15 cases of normal gastric mucosa. RESULTS: The expression of PTEN in gastric cancer was low (28/54, 51.9%), and reduced with the increased invasive depth (P = 0.004) and clinical stages (P = 0.001), reduced histological differentiation (P = 0.008) and occurrence of lymph node (P = 0.003) and long distance metastasis (P = 0.015). The expression of MMP-9 in gastric cancer was high (41/54, 75.9%), and increased with increased invasive depth (P = 0.04) and clinical stages (P = 0.039), reduced his-tological differentiation (P = 0.009), and occurrence of lymph node metastasis (P = 0.025). The expression of Caspase-3 in gastric cancers was low (12/54, 22.2%), and reduced with increased clinical stages (P = 0.015), reduced histological differentiation (P = 0.035) and occurrence of lymph node metastasis (P = 0.045). The expression of PTEN and MMP-9 (r = -0.543, P = 0.001), Caspase-3 and MMP-9 (r = -0.741, P = 0.001) were in inversely correlation, while the expression of PTEN and Caspase-3 (r = 0.515, P = 0.001) were in positive correlation in gastric cancer. CONCLUSION: PTEN and Caspase-3 are lowly expressed in gastric caner, while MMP-9 is highly expressed. PTEN, MMP-9, and Caspase-3 can serve as the markers in the diagnosis and prognosis of gastric cancer.