| 热休克蛋白70在哮喘小鼠气道炎症模型中的表达及意义 |
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| 引用本文： | 滕茂蓉,葛海燕,倪松石.热休克蛋白70在哮喘小鼠气道炎症模型中的表达及意义[J].江苏医药,2008,34(5):490-493. |
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| 作者姓名： | 滕茂蓉 葛海燕 倪松石 |
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| 作者单位： | 1. 泰州市人民医院老年医学科
2. 南通大学附属医院呼吸病科,226001 |
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| 基金项目： | 南通大学校科研和教改项目 |
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| 摘 要： | 目的 观察哮喘小鼠肺组织中热休克蛋白70(HSP70)的表达及其在哮喘中的作用.方法 将32只BALB/c小鼠随机分为N(正常对照组)、A(急性哮喘组I)、B(急性哮喘组II)和C(慢性哮喘组)4组,每组8只;卵蛋白致敏和激发建立哮喘小鼠模型;支气管肺泡灌洗液(BALF)分析和HE染色鉴定哮喘模型;免疫组化及Western blot法测定各组小鼠肺组织中HSP70的表达和定位.结果 哮喘组BALF嗜酸性粒细胞绝对计数及分类计数较N组皆有显著增高(P＜0.05),HE染色提示哮喘组与N组相比出现嗜酸性粒细胞浸润增多、纤毛脱失、平滑肌细胞层增厚等改变.HSP70在哮喘各组小鼠中主要定位在支气管上皮细胞、部分肺泡上皮细胞和炎症细胞中,而在N组中表达呈阴性结果;HSP70在哮喘组表达量较N组为高(P＜0.05),B组表达量显著高于A组(P＜0.05).结论 哮喘小鼠肺组织中HSP70主要表达于支气管上皮细胞、肺泡上皮细胞和炎症细胞中,参与哮喘发病.
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| 关 键 词： | 热休克蛋白70 支气管哮喘 |
| 文章编号： | 0253-3685(2008)05-0490-03 |
| 修稿时间： | 2007年12月28 |
Expression and role of heat shock protein 70 in a mouse bronchial asthma model |
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| TENG Maorong,GE Haiyan,NI Songshi.Expression and role of heat shock protein 70 in a mouse bronchial asthma model[J].Jiangsu Medical Journal,2008,34(5):490-493. |
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| Authors: | TENG Maorong GE Haiyan NI Songshi |
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| Institution: | TENG Maorong,GE Haiyan,NI Songshi.Department of Respiratory Diseases,Affiliated Hospital,Nantong University,Nantong 226001,CHINA |
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| Abstract: | Objective To evaluate the expression and role of heat shock protein 70(HSP70) in asthmatic mice lung. Methods Thirty-two BALB/c mice were randomized to group N,group A,group B and group C with 8 mice each. Mice were sensitized and challenged with ovalbumin to establish the asthmatic model. The multiple indices of cytology and pathomorphology were measured by bronchoalveolar lavage fluid(BALF) analysis and HE staining to confirm the existence of asthma and the characters of airway inflammation. Pulmonary exp... |
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| Keywords: | Heat shock protein 70 Bronchial asthma |
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