E-选择素在慢性阻塞性肺疾病大鼠支气管肺组织中的表达

目的制备慢性阻塞性肺疾病（COPD）大鼠模型,探讨E-选择素在COPD气道炎症中的作用.方法采用两次气管内注入脂多糖（LPS）和反复烟熏的方法制作COPD大鼠模型（B组）;采用免疫组化法检测E-选择素在COPD大鼠支气管肺组织中的表达.结果所建大鼠模型的病理及病理生理学改变基本符合人类COPD的特点;模型组支气管肺组织微小血管内皮细胞E-选择素的表达强度显著高于对照组（A组） （P〈0.01）,且与支气管肺泡灌洗液（BALF）中白细胞总数及中性粒细胞数成显著正相关（分别为：r=0.82,P〈0.001;r=0.79,P〈0.001）. 结论 E-选择素可能参与了COPD气道炎症过程.

The expression of E-selectin on bronchpulmonary tissues of a rat model of chronic obstructive pulmonary disease

Objective To study the role of E-selectin in the airway inflammation in a rat modle of chronic obstructive pulmonary disease(COPD).Methods COPD rat model(B group) was established by intracheal instillation of lipopolysacharide(LPS) twice and exposure to cigarette smoke daily;the expression of E-selectin on rat lung tissue was detected by means of immunohistochemistry(sp).Results The changes in histopathology and pathophysiology in the animal model were similar to those in COPD patients;The expression of E-selectin on bronchpulmonary capillary endothelial cells of the model group was significantly increased compared to the control group(A group)(P<0.01)and positively correlated with the leukocyte numbers and neutrophils in BALF in the model group(r=0.82,P<0.001;r=0.79,P<0.001,respectively).Conclusion E-selectin may be involved in the process of airway inflammation in COPD.