| 强稳恒磁场对离体牛肝过氧化氢酶构象及活力的影响 |
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| 引用本文： | 张军,孙凡,陈德万,李光林. 强稳恒磁场对离体牛肝过氧化氢酶构象及活力的影响[J]. 中国医学物理学杂志, 2001, 18(1): 35-36,53 |
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| 作者姓名： | 张军 孙凡 陈德万 李光林 |
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| 作者单位： | 1. 重庆医科大学生物教研室,
2. 西南农业大学物理教研室, |
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| 摘 要： | 目的研究了强稳恒磁场对离体牛肝过氧化氢酶(CAT)构象及活力的影响。材料与方法将天然酶液置于不同磁感应强度的磁场中处理不同的时间撤离磁场后立即对其进行光谱分析并用Beers&Sizers法(改良型)测定酶活力。结果磁场使过氧化氢酶的构象发生变化,表现为λ=210～310nm的紫外差光谱以及λ=330nm荧光发射峰的荧光强度改变构象变化的同时酶活力增加。结论磁场对酶分子的影响可能是导致机体宏观磁效应产生的重要原因。
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| 关 键 词： | 过氧化氢酶 稳恒磁场 光谱分析 酶活测定 |
| 文章编号： | 1005-202X(2001)01-0035-02 |
Studies of biologicaleffects of high intensities static magnetic fields on the conformation and activity ofvitro catalase in bovine liver |
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| ZHANG Jun,SHUN Fan,CHEN De-wan,et al.. Studies of biologicaleffects of high intensities static magnetic fields on the conformation and activity ofvitro catalase in bovine liver[J]. Chinese Journal of Medical Physics, 2001, 18(1): 35-36,53 |
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| Authors: | ZHANG Jun SHUN Fan CHEN De-wan et al. |
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| Abstract: | purpose: Studied the effects of static magnetic fields on vitro catalase's conformation and activity, method: After the natural enzyme was exposed under various intensities of magnetic fields for a certain period of time, got it out of magnetic fields at once , then analyzed its spectra and assayed its activity, result: The conformation of magnetized catalase changed to a certain extent, which led to y=210-310nm ultraviolet differential spectra and variation of fluorescence intensity at y=330 nm emission peak; The activity increased as well with the conformational change, conclusion: Magnetic effects of enzymatic molecular might be the key cause that resulted in organism biological effects. |
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| Keywords: | catalase static magnetic fields spectra analyses enzymatic activity assay |
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