下丘脑-垂体轴反馈路径对内皮细胞NO和ICAM-1表达的影响

目的：探讨下丘脑-垂体轴反馈调节对动脉粥样硬化形成的影响。方法：将OX-LDL损伤内皮细胞的信息分别反馈于下丘脑（H）、垂体（P）、下丘脑-垂体（HP）,制成下丘脑-垂体轴条件培养液再作用于受损内皮细胞,检测内皮细胞一氧化氮（NO）产量和细胞间粘附分子-1（ICAM-1）的表达。结果：H组和P组条件培养液对受损内皮细胞NO产量、ICAM-1表达的作用不明显（P〉0.05）,HP组能使其NO产量显著增加（P〈0.01）,同时减少ICAM-1的表达（P〈0.05）。结论：完整的下丘脑-垂体轴对内皮细胞NO代谢、ICAM-1的表达有反馈调控作用,可抑制AS形成。

Influence of the feedback ways of hypothalamus-pituitary axis on NO and ICAM-1 of cultured endothelial cells

Objective：To investigate the feedback of hypothalamus-pituitary axis influencing the development of atherosclerosis.Methods：The hypothalamus（H）、pituitary（P） and hypothalamus-pituitary cells（HP） were cultured respectively with feedback message from endothelial cells induced by OX-LDL,then the conditioned media were used to culture damaged endothelial cells,and the supernatants were collected separately for detecting the content of NO and the endothelial cells were stained by immunocytochemical method to observe the expression of ICAM-1.Results：It was shown that the conditioned media of H and P groups had no effect on the metabolism of NO and ICAM-1 expression of endothelial cells（P0.05）,but the media of HP group intensively up-regulated the metabolism of NO（P0.01） and down-regulated the ICAM-1 expression（P0.05）.Conclusion：The unbroken hypothalamus-pituitary axis with feedback message can regulate the metabolism of NO and ICAM-1 expression of endothelial cells,which can restrain the atherosclerosis.