髓样分化因子MyD88在乳腺癌与癌旁正常组织中的表达与意义

目的探讨髓样分化因子MyD88(myeloid differentiation factor 88,MyD88)在乳腺癌及癌旁正常组织中的表达特点及分布。方法收集本科及合川区人民医院普外科35例乳腺癌患者的临床病理资料(2010年1月至2011年9月),平均年龄52岁。应用RT-PCR、Western blot方法检测MyD88在乳腺癌及癌旁正常组织中的基因和蛋白表达水平;应用免疫组化等方法检测MyD88在乳腺癌及癌旁正常组织结构中的分布,观察在该组织中的表达特点和变化规律。结果 RT-PCR结果显示,与癌旁正常组织相比,MyD88在乳腺癌组织中表达明显增高(P<0.01);Western blot结果显示,与癌旁正常组织相比,MyD88在乳腺癌组织中表达明显增高(P<0.01);MyD88蛋白在乳腺癌周围正常组织中也有部分表达,部分细胞呈弱阳性表达,在乳腺癌组织则呈现明显强烈高表达。不同细胞类型差异性表达MyD88,且与肿瘤的TNM分期、肿瘤大小、淋巴侵袭转移密切相关。结论 MyD88可能在乳腺癌病理发生、发展过程中起到一定的调控作用。

Expression and significance of myeloid differentiation factor 88 in breast carcinomas and adjacent normal tissues

Objective To determine the expression profile and distribution of myeloid differentiation factor 88(MyD88) in breast carcinomas and adjacent normal tissues to investigate the role of innate immune factors downstream signaling in the breast carcinoma.Methods Clinical data of 35 cases of pathologically diagnosed breast carcinomas who received radical resection in our department during January 2010 to September 2011 were collected in this study.RT-PCR and Western blot analysis were used to detect the expression of MyD88 at mRNA and protein levels in the breast carcinomas and adjacent normal tissue.Immunohistochemical assay was employed to study the distribution of MyD88 in these tissues for its expression profile and variation.Results RT-PCR showed that the expression rate of MyD88 was significantly increased in breast carcinoma,with statistically significant compared to adjacent normal tissues(P<0.01).Western blot analysis displayed similar results in protein expression of MyD88 in the two tissues(P<0.01).MyD88 was also mildly expressed in adjacent normal tissues,with some cells displaying weakly positive expression.In contrast,breast carcinoma tissue showed strong expression.The expression of MyD88 was differential in different cell types,and was closely related to TNM stages,tumor size,lymphatic invasion and metastasis.Conclusion MyD88 may play a regulatory role in breast cancer incidence and development process.