“劳倦过度、房室不节”肾阳虚模型小鼠免疫功能的观察

目的:观察“劳倦过度、房室不节”诱发的肾阳虚模型小鼠免疫功能的变化。方法:选健康雄性昆明种小白鼠225只,随机分为5组:对照组、劳倦过度组、房室不节组、劳倦过度+房室不节组(模型组)和治疗组各45只。用强迫小鼠游泳方法造成劳倦过度;以Colldege效应诱导雄性小鼠房室不节,建立肾阳虚小鼠模型,用金匮肾气丸作为治疗药物,观察各组胸腺指数、脾指数I、L-2及淋巴细胞转化率等免疫指标的变化。结果:模型组各项指标均较对照组明显下降(P<0.05);治疗组各项指标较模型组明显升高(P<0.05)。结论:进一步证实中医肾在免疫调节中具有主导作用。

Observation on immune function of mouse models of deficiency of kidney-Yang induced by overfatigue and sexual overstrain

Objective: To observe changes of immune function in mice models of deficiency of kidney-yang induced by overfatigue and sexual overstrain.Methods: 225 healthy male mice were categorized into five groups randomly: control group(45),overfatigue group(45),sexual overstrain group(45),overfatigue plus sexual overstrain group(model group)(45),therapy group(45).Overfatigue model could be made by forcing mice to swim and deficiency of kidney-Yang by colldege effect to induce sexual overstrain in male mice.Jingui Shenqi pills could serve as medication.Changes of immune indexes(thymus index, spleen index,IL-2,lymphocyte transformation test) were examined in different groups.Results: Immune indexes in model group were lower than those in control group(P<0.05)while they were higher in therapy group(P<0.05),compared with those in model group.Conclusion: It is further proved that kidney places a leading role in the immuno-regulation.