心肌内注射碱性成纤维细胞生长因子基因缩小兔缺血心肌面积的实验研究

目的　探讨心肌内注射碱性成纤维细胞生长因子基因 (bFGF基因 )缩小兔急性心肌缺血面积的作用。方法　碱裂解法大量制备质粒 ,采用开胸扎兔冠状动脉左前降支 (LAD)法 ,建立兔急性前壁心肌梗死模型 ,模型制备成功后将动物分为治疗组 (n =1 9)和对照组 (n =1 8) ,并于心肌内分别注射pcDNA3 -bFGF1 0 0 μg ,和pcDNA3 1 0 0 μg ,饲养至 2、 6、 1 2周处死 ,行病理切片观察心肌梗死面积的变化和缺血心肌内血管新生的情况。结果　 (1 )术前术后描记的心电图证实兔急性心肌梗死模型制作成功。 (2 )病理切片行图象分析计算血管密度发现 ,治疗组毛细血管密度和小动脉密度显著高于对照组。 (3)治疗组 2周和 6周缺血心肌面积显著小于对照组。结论　心肌内注射bFGF基因能促进缺血心肌内血管新生、减少心肌缺血面积 ,基因治疗有望成为一种新的冠心病治疗方法

The experimental study of intramyocardial injection of naked DNA encoding bFGF for decreasing size of ischemic myocardium in rabbits.

Objective To study whether intramyocardial injection of naked DNA encoding bFGF can decrease the size of ischemic myocardium in rabbits model.Method 42 rabbits underwent left thoracotomy followed by the ligation of left anterior descending coronary artery. After model reproducting, the rabbits were randomized to receive a directly intramyocardial injection of either pcDNA3 bFGF(n=19) or pcDNA3(n=18). 2, 6 or 12 weeks later, histologic analysis was performed to evaluate angiogenesis and ischemic size induced by gene therapy.Result Changes of electrocardiogram testified that the model of AMI was successful. Histologic analysis showed there was a significant increase in the density of capillaries and arterioles, and ischemic size decreased significantly in gene therapy group. Conclusion Directly intramyocardial injection of bFGF gene can reduce the size of ischemic myocardium and promote angiogenesis in ischemic myocardium. It suggests that bFGF gene may have the possibility to become a new treatment strategy for coronary heart disease.