| 蛇血清分泌型磷脂酶A_2抑制剂防治人炎症的生物信息学分析 |
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| 引用本文： | 陈柯,邓欣如,徐曦,李晓,钟立鹏,黄春洪. 蛇血清分泌型磷脂酶A_2抑制剂防治人炎症的生物信息学分析[J]. 基础医学与临床, 2011, 31(9) |
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| 作者姓名： | 陈柯 邓欣如 徐曦 李晓 钟立鹏 黄春洪 |
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| 作者单位： | 南昌大学,基础医学院,生物化学教研室,江西,南昌,330006 |
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| 基金项目： | 国家大学生创新实验计划(091040303); 国家自然科学基金(30860347) |
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| 摘 要： | 目的比较人和蛇毒分泌型磷脂酶A2(sPLA2)结构相似性,分析蛇血清磷脂酶A2抑制剂(PLI)对sPLA2特异性抑制的结构与功能关系,评价蛇血清PLI对人炎症的抑制作用。方法用Clustal W2对人和五步蛇sPLA2氨基酸序列进行多重序列比对。用Swiss-model对各种sPLA2的空间结构进行同源建模预测,并用Chimera软件对人和蛇毒sPLA2空间结构进行三维比对。结果人和五步蛇sPLA2一级序列同源性有40%左右,空间结构几乎相似。各种PLI具有相同的空间结构特征和保守的sPLA2结合区。结论理论上推断蛇血PLI可有效抑制人sPLA2活性,减少sPLA2介导的炎症发生。
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| 关 键 词： | 磷脂酶A2 磷脂酶A2抑制剂 生物信息学 |
Bioinformatics analysis of possible inhibition of human inflammation by secretory phospholipase A_2 inhibitor from snake serum |
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| CHEN Ke,DENG Xin-ru,XU Xi,LI Xiao,ZHONG Li-peng,HUANG Chun-hong. Bioinformatics analysis of possible inhibition of human inflammation by secretory phospholipase A_2 inhibitor from snake serum[J]. Basic Medical Sciences and Clinics, 2011, 31(9) |
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| Authors: | CHEN Ke DENG Xin-ru XU Xi LI Xiao ZHONG Li-peng HUANG Chun-hong |
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| Affiliation: | CHEN Ke,DENG Xin-ru,XU Xi,LI Xiao,ZHONG Li-peng,HUANG Chun-hong(Dept of Biochemistry,College of Basic Medical Sciences,Nanchang University,Nanchang 330006,China) |
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| Abstract: | Objective To compare the structural similarities of human secretory phospholipase A2(sPLA2) with snake venom sPLA2,and to analyze the neutralization effect of sPLA2 inhibitors(PLIs) from snake serum against sPLA2 by exploring their relationship between structure and function,which was meaningful for assessing PLIs' clinical application in inflammation protection.Methods Multiple sequencial alignment of 7 human sPLA2 and 3 five-pace snake venom sPLA2 was made by Clustal W2.Tertiary structures of the 10 sPLA2... |
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| Keywords: | phospholipase A2 phospholipase A2 inhibitor bioinformatics |
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