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| PI3K/AKT通路介导H_2O_2预处理减轻PC12细胞氧化损伤 | |
| 引用本文： | 张梅,李卫,郭瑞鲜,罗健东,冯鉴强.PI3K/AKT通路介导H_2O_2预处理减轻PC12细胞氧化损伤[J].基础医学与临床,2011,31(8). |
| 作者姓名： | 张梅 李卫 郭瑞鲜 罗健东 冯鉴强 |
| 作者单位： | 1. 广州医学院药理教研室,广东广州,510082 2. 暨南大学附属第一医院神经外科,广东广州,510630 3. 中山大学中山医学院生理教研室,广东广州,510080 |
| 基金项目： | 广州医学院博士启动基金(200922); 广州市教育局科研基金(10A178) |
| 摘 要： | 目的探讨PI3K/AKT信号通路是否参与H2O2预处理诱导的适应性细胞保护作用。方法体外培养PC12细胞,建立H2O2预处理对抗高浓度H2O2诱导细胞损伤的实验模型。应用甲氮甲唑蓝(MTT)法测定细胞的存活率,比色法测定乳酸脱氢酶(LDH)的活性,碘化丙啶(PI)染色流式细胞术检测细胞凋亡率,免疫印迹法(Westernblot)测定AKT的表达。结果 100μmol H2O2预处理PC12细胞90 min可显著地抑制300μmol H2O2引起的损伤,使细胞存活率从50.2%±4.6%升高至83.8%±3.5%,LDH活性由103%±10.2%下降至68.5%±5.3%,细胞凋亡率由65.5%±4.1%下降至37.1%±2.3%(P<0.01)。100μmol H2O2预处理诱导p-AKT的表达,PI3K抑制剂ly294002阻断了H2O2预处理引起的p-AKT表达。同时ly294002拮抗了H2O2预处理诱导的抗细胞损伤和凋亡作用。结论 H2O2预处理通过PI3K途径引起AKT的活化,PI3K/AKT通路的活化介导了H2O2预处理诱导的适应性细胞保护作用。 |
| 关 键 词： | 过氧化氢 预处理 PI3K/AKT信号通路 细胞保护 |
PI3K/AKT pathway mediates the anti-oxidative injury induced by H_2O_2 preconditioning in PC12 cell |
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| ZHANG Mei,LI Wei,GUO Rui-xian,LUO Jian-dong,FENG Jian-qiang.PI3K/AKT pathway mediates the anti-oxidative injury induced by H_2O_2 preconditioning in PC12 cell[J].Basic Medical Sciences and Clinics,2011,31(8). | |
| Authors: | ZHANG Mei LI Wei GUO Rui-xian LUO Jian-dong FENG Jian-qiang |
| Institution: | ZHANG Mei1,LI Wei2,GUO Rui-xian3,LUO Jian-dong1,FENG Jian-qiang3(1.Dept.of Pharmacology,Guangzhou Medical College,Guangzhou 510082,2.Dept.of Neurosurgery,the First Affiliated Hospital,Jinan University,Guangzhou 510630,3.Dept.of Physiology,Zhongshan Medical College,Sun Yat-sen University,Guangzhou 510080,China) |
| Abstract: | Objective To explore whether PI3K/AKT pathway plays a role in H2O2 preconditioning-induced adaptive cytoprotection.Methods Setting up an experimental model of H2O2 preconditioning after injury induced by H2O2 at high concentration in cultured PC12 cells.The viability of cells was measured by MTT assay.The activity of lactate dehydrogenase(LDH) was assessed by colorimetry.The percentage of apoptotic cells was measured by flow cytometry(FCM) with propidium iodide stain.AKT were detected by Western blot.Result... |
| Keywords: | hydrogen peroxide precondition PI3K/AKT pathway cytoprotection |
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