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| TRAIL诱导Hela细胞凋亡的线粒体信号通路 | |
| 引用本文： | 叶记林,刘永春,于有江,刘延庆.TRAIL诱导Hela细胞凋亡的线粒体信号通路[J].基础医学与临床,2011,31(10):1120-1123. |
| 作者姓名： | 叶记林 刘永春 于有江 刘延庆 |
| 作者单位： | 1. 扬州环境资源职业技术学院医学系,江苏扬州,225127 2. 江苏省苏北人民医院,江苏扬州,225000 3. 扬州大学医学院,江苏扬州,225001 |
| 基金项目： | 扬州环境资源职业技术学院基金(07001) |
| 摘 要： | 目的 探讨 TRAIL 诱导人宫颈癌 Hela 细胞凋亡的线粒体通路。 方法 琼脂糖凝胶电泳判断细胞凋亡；激光共聚焦、Western blot、荧光免疫和 caspase-3 活性检测测定细胞线粒体膜电位 (?Ψm) 、Bcl-2 蛋白、细胞色素 c (Cyt c) 和凋亡诱导因子 (AIF) 蛋白在细胞中的定位以及 caspase-3 活性。结果 TRAIL 能诱导 Hela 细胞凋亡，有凋亡细胞特有的 DNA 梯状条带。同时，TRAIL具有时间依赖性致 ?Ψm 和 Bcl-2 蛋白含量明显下降，线粒体 Cyt c 蛋白释放，AIF 蛋白向细胞质、细胞核转移，caspase-3 活性增强。结论 TRAIL 诱导 Hela 细胞凋亡途径之一是通过线粒体信号通路进行的。 |
| 关 键 词： | TRAIL Hela 细胞 细胞凋亡 线粒体 |
TRAIL induced apoptosis in Hela cells by mitochondrial signal pathway |
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| YE Ji-lin,LIU Yong-chun,YU You-jiang,LIU Yan-qing.TRAIL induced apoptosis in Hela cells by mitochondrial signal pathway[J].Basic Medical Sciences and Clinics,2011,31(10):1120-1123. | |
| Authors: | YE Ji-lin LIU Yong-chun YU You-jiang LIU Yan-qing |
| Institution: | YE Ji-lin1,LIU Yong-chun2,YU You-jiang1,LIU Yan-qing3(1.Medical Science Department,Yangzhou Vocational College of Environment and Resources,Yangzhou 225127,2.Subei People's Hospital of Jiangsu Province,Yangzhou 225000,3.Medical Academy,Yangzhou University,Yangzhou 225001,China) |
| Abstract: | Objective To explore the mitochondrial pathway in the apoptosis of Hela cells induced by TRAIL.Methods Apoptotic cells were detected by DNA electrophoresis.The mitochondria membrane potential(MMP),the expressions of BCL-2,the location of Cyt c and AIF,the caspase-3 activity were tested by confocal laser microscopy,western blot,immunofluorescence and caspase-3 activity assay.Results TRAIL can induce apoptosis in Hela cells.DNA ladders were showed on agarose gel electrophoresis.At the same time,TRAIL induced ... |
| Keywords: | TRAIL hela cel1s apoptosis mitochondria |
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