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| 细丝蛋白A抑制人结肠癌SW480细胞体外侵袭转移能力 | |
| 引用本文： | 史建伟,于跃明,王士杰,杨珊,孔繁龙,张娟,王贵英. 细丝蛋白A抑制人结肠癌SW480细胞体外侵袭转移能力[J]. 基础医学与临床, 2011, 31(9): 1000-1005 |
| 作者姓名： | 史建伟 于跃明 王士杰 杨珊 孔繁龙 张娟 王贵英 |
| 作者单位： | 1. 河北大学,附属医院,肿瘤外科,河北,保定,071000;河北医科大学,第四医院外二科,河北,石家庄,050011 2. 河北医科大学,第四医院外二科,河北,石家庄,050011 3. 河北医科大学,第四医院肿瘤研究所,河北,石家庄,050011 4. 河北医科大学,第四医院外一科,河北,石家庄,050011 5. 山西医科大学,临床二系07级,山西,太原,030001 |
| 基金项目： | 河北省卫生厅医学科学研究重大课题(20090010); 河北省普通高校强势特色学科建设基金(200552) |
| 摘 要： | 摘要 目的 研究细丝蛋白A（filamin A,FLNa）对人结肠癌SW480细胞侵袭转移行为的影响，并初步探讨其抑癌的机制。方法 将FLNa基因质粒载体pcDNA3.1/V5-His-TOPO/FLNa，以脂质体介导方式转染SW480细胞。经G418筛选，获得FLNa基因稳定表达的SW480 /FLNa细胞。逆转录聚合酶链反应（RT-PCR）和Western blot技术检测FLNa基因的导入情况；RT-PCR和Western blot检测了SW480细胞中基质金属蛋白酶-9（matrix metalloproteinases-9,MMP-9）的表达水平；MTT法检测细胞的增殖能力；划痕损伤实验、Transwell小室和Matrigel侵袭模型评价FLNa对细胞侵袭和转移能力的影响。结果 RT-PCR和Western blot证实FLNa的表达载体pcDNA3.1/V5-His -TOPO/FLNa在SW480细胞中获得稳定表达；SW480/FLNa细胞MMP-9的mRNA和蛋白表达水平低于野生型SW480细胞；划痕损伤实验、Transwell小室和Matrigel侵袭模型表明FLNa抑制SW480细胞的侵袭和转移。结论 FLNa能够抑制SW480细胞的侵袭和转移能力，其机制可能与降低MMP-9的表达有关。 |
| 关 键 词： | 结肠肿瘤 细丝蛋白A 侵袭 转移 |
Inhibitory effect of filamin A on invasion and metastasis of human colon carcinoma cell line SW480 in vitro |
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| SHI Jian-wei,YU Yue-ming,WANG Shi-jie,YANG Shan,KONG Fan-long,ZHANG Juan,WANG Gui-ying. Inhibitory effect of filamin A on invasion and metastasis of human colon carcinoma cell line SW480 in vitro[J]. Basic Medical Sciences and Clinics, 2011, 31(9): 1000-1005 | |
| Authors: | SHI Jian-wei YU Yue-ming WANG Shi-jie YANG Shan KONG Fan-long ZHANG Juan WANG Gui-ying |
| Affiliation: | SHI Jian-wei1,2,YU Yue-ming2,WANG Shi-jie3,YANG Shan4,KONG Fan-long5,ZHANG Juan2,WANG Gui-ying2(1.Dept.of Oncologic Surgery,the Affiliated Hospital of Hebei University,Baoding 071000,2.Second Dept.of Surgery,3.Cancer Institute,4.First Dept.of Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,5.Grade 07 of Second Dept.of ClinicalMedicine,Shanxi Medical University,Taiyuan 030001,China) |
| Abstract: | Objective To explore the inhibitory effect of Filamin A(FLNa) on invasion and metastasis of human colon carcinoma cell line SW480 and investigate its mechanisms.Methods Expressing vector pcDNA3.1/V5-His-TOPO/ FLNa was transfected into SW480 cells by lipofectamine protocols.After G418 selection,the SW480/FLNa cells expressing FLNa stably were obtained.Expression of FLNa in tumor cells was confirmed by RT-PCR and Western blot.RT-PCR assay and Western blot were also employed to detect the expression of matrix ... |
| Keywords: | colon neoplasms filamin A invasion metastasis |
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