| 脑缺血/再灌注致神经元退行性变进程中自由基水平和SOD2表达相关 |
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| 引用本文： | 袁野,杨俊卿,周岐新.脑缺血/再灌注致神经元退行性变进程中自由基水平和SOD2表达相关[J].基础医学与临床,2011,31(11):1229-1233. |
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| 作者姓名： | 袁野 杨俊卿 周岐新 |
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| 作者单位： | 重庆医科大学药学院药理学教研室,重庆,400016 |
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| 基金项目： | 国家自然科学基金(30672211) |
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| 摘 要： | 目的 探讨脑缺血/再灌注(I/R)致神经元退行性变所涉及的自由基和SOD2表达的时程变化及其相互关系。方法 抽取并回输约40%总血量加双侧颈总动脉夹闭20 min建立I/R损伤模型。组织学检测神经元损伤；Morris水迷宫检测学习记忆能力；分光光度计法检测SOD2活性和丙二醛(MDA)含量；Western blot检测SOD2蛋白表达。结果 与假手术组相比，I/R组小鼠学习记忆能力显著降低，海马MDA含量进行性显著升高（P<0.05）；I/R术后5、15、30和60 d组海马SOD2活性（14.69+2.49 U/mg.pro，14.15+2.41 U/mg.pro，15.07+2.67 U/mg.pro，15.55+2.64 U/mg.pro）和蛋白表达（9.20+1.23，5.40+1.03，4.90+0.92，5.60+0.59）均显著低于假手术组（P<0.05）；海马出现进行性神经元核固缩加重和神经元丢失。结论 I/R致小鼠神经元退行性变与SOD2蛋白表达和活性保持低水平状态而氧化应激进行性增强有关。
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| 关 键 词： | 缺血/再灌注 神经元退行性变 超氧化物歧化酶-2 自由基 |
Relationship between SOD2 expression and free radical level in neurodegeneration induced by ischemia-reperfusion in mouse |
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| YUAN Ye,YANG Jun-qing,Zhou Qi-xin.Relationship between SOD2 expression and free radical level in neurodegeneration induced by ischemia-reperfusion in mouse[J].Basic Medical Sciences and Clinics,2011,31(11):1229-1233. |
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| Authors: | YUAN Ye YANG Jun-qing Zhou Qi-xin |
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| Institution: | YUAN Ye,YANG Jun-qing,Zhou Qi-xin*(Dept.of Pharmacology,Institute of Pharmacy,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the changes of time process of SOD2 and free radicals in neurodegeneration induced by cerebral ischemia-reperfusion(I/R).Methods Cerebral I/R model was established through drawing out and reperfusing 40% of the whole blood volume in combination with clamping the carotid arteries for 20 min.Histological observation was performed to evaluate the neural damage;learning and memory ability was examined by Morris water maze;SOD2 activity and malonaldehyde(MDA) were determined by spectroph... |
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| Keywords: | ischemia-reperfusion neurodegeneration superoxide dismutase-2 free radical |
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