骨髓间充质干细胞对衰老大鼠肠损伤修复作用的研究

 目的 探讨骨髓间充质干细胞（MSCs）在D-半乳糖制备的衰老大鼠小肠损伤中的作用。方法SD大鼠30只,随机均分为3组：对照组、衰老模型组和MSCs防治组。给衰老模型组大鼠每日皮下注射D-半乳糖400mg/kg,连续4个月。MSCs防治组在衰老模型制备成功后,给予尾静脉输注3×106 个MSCs。分别采用硫代巴比妥酸和黄嘌呤氧化法检测小肠组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;观察3组大鼠小肠组织结构的差异,并用表达绿色荧光蛋白（GFP）的慢病毒载体标记MSCs,以确定MSCs的植入情况。结果 GFP标记的MSCs移植给大鼠后,能向小肠组织迁移并存活。与衰老模型组相比,MSCs防治组SOD 的活性明显升高,MDA含量明显降低,差异有显著性意义,分别为：（133.7±3.6）,（105.1±4.3）U/ml,P <0.01;（5.9±0.1）,（6.9±0.1）(nmol/ml),P <0.01。模型组大鼠肠道粘膜损伤严重,而MSCs防治组大鼠的小肠损伤有明显修复。结论 骨髓间充质干细胞能够一定程度上减轻D-半乳糖致衰老大鼠的小肠损伤。

Bone marrow mesenchymal stem cells transplantation for the reduction of intestine injury in aging rat

Objective To observe the effect of bone-marrow mesenchymal stem cells on intestine in the aging rats induced by D-galactose.Methods The aging model group were injected with D-galactose for 4 months to establish the aging rats.The contents of superoxide dismutase(SOD) and malondialdehyde(MDA) in intestine was measured wtih hydroxylamine and chromatometry respectively.The pathological morphological changes of intestine were observed.MSCs were transfected by adenovirus vectors expressing green fluorescent prot...