| 1,25-二羟维生素D_3减轻哮喘小鼠的气道重塑 |
| |
| 引用本文： | 宋颖芳,赖国祥,戚好文,吴昌归. 1,25-二羟维生素D_3减轻哮喘小鼠的气道重塑[J]. 基础医学与临床, 2011, 31(9) |
| |
| 作者姓名： | 宋颖芳 赖国祥 戚好文 吴昌归 |
| |
| 作者单位： | 1. 南京军区福州总医院,呼吸与危重症医学科,福建,福州,350025
2. 第四军医大学,西京医院,呼吸内科,陕西,西安,710032 |
| |
| 摘 要： | 目的探讨1,25-二羟维生素D3(1,25(OH)2D3,VD)对哮喘小鼠气道重塑及其肺组织中基质金属蛋白酶-9(MMP-9)表达的干预作用及其机制。方法建立慢性哮喘小鼠模型,将小鼠随机分为对照组、哮喘组、地塞米松(DEX)组及VD组。HE染色观察各组气道结构;用计算机图像分析系统评价各组气道重塑;用明胶酶谱法及RT-PCR法检测各组的MMP-9的活性及其mRNA表达。结果 (1)哮喘组出现炎性细胞浸润增多、上皮细胞脱落及平滑肌细胞层增厚等气道重塑改变,而DEX组及VD组均可部分逆转上述病理改变;(2)DEX组及VD组的支气管内壁厚度、平滑肌层厚度和平滑肌细胞核数显著低于哮喘组,但仍高于对照组(P<0.05);(3)DEX组及VD组肺组织MMP-9的活性分别为对照组的(2.24±0.16)倍及(3.46±0.09)倍,而哮喘组是对照组的(7.87±0.09)倍(P<0.05);(4)各组MMP-9mRNA相对定量分别为对照组(0.57±0.08)、哮喘组(5.74±0.13)、DEX组(2.63±0.11)及VD组(3.16±0.09),且两两比较均P<0.05。结论 1,25(OH)2D3可显著减轻哮喘气道...
|
| 关 键 词： | 哮喘 气道重塑 1 25-二羟维生素D3 地塞米松 基质金属蛋白酶-9 |
1,25(OH)_2D_3 could alleviate airway remodeling in model murine of chronic asthma |
| |
| SONG Ying-fang,LAI Guo-xiang,Qi Hao-wen,WU Chang-gui. 1,25(OH)_2D_3 could alleviate airway remodeling in model murine of chronic asthma[J]. Basic Medical Sciences and Clinics, 2011, 31(9) |
| |
| Authors: | SONG Ying-fang LAI Guo-xiang Qi Hao-wen WU Chang-gui |
| |
| Affiliation: | SONG Ying-fang1,LAI Guo-xiang1,QI Hao-wen2,WU Chang-gui2(1.Division of Pulmonary and Critical Care Medicine,Fuzhou General Hospital of Najing Military Command,Fuzhou 350025,2.Dept.of Respiratory Disease,Xijing Hospital,the 4th Military University,Xi'an 710033,China) |
| |
| Abstract: | Objective To investigate the effects of 1,25(OH)2D3 on airway remodeling and the expression of matrix metalloprotease-9(MMP-9) in a murine model of chronic asthma,and to explore the potential mechanism of 1,25(OH)2D3 in the treatment of asthma.Methods BALB/c mice were sensitized and challenged with ovalbumin to establish the chronic asthmatic model.They were randomly divided into control group,asthma group,dexamethasone(DEX) group and VD group.The characteristic airway inflammation and alteration of airway ... |
| |
| Keywords: | asthma airway remodeling 1 25(OH)2D3 dexamethasone matrix metallo-protease-9 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
