| 细粒棘球绦虫重组Bb-Eg95-EgA31疫苗诱导BALB/c小鼠的保护力观察 |
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| 引用本文： | 周必英,陈雅棠,李文桂,杨梅. 细粒棘球绦虫重组Bb-Eg95-EgA31疫苗诱导BALB/c小鼠的保护力观察[J]. 中国病原生物学杂志, 2010, 0(2) |
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| 作者姓名： | 周必英 陈雅棠 李文桂 杨梅 |
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| 作者单位： | 重庆医科大学附属第一医院传染病寄生虫病研究所;贵州省遵义医学院寄生虫学教研室; |
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| 基金项目： | 国家自然科学基金项目(No.30801052,30671835,30500423和30200239) |
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| 摘 要： | 目的探讨细粒棘球绦虫(Eg)重组双歧杆菌(Bb)-Eg95-EgA31疫苗免疫和Eg原头节攻击后小鼠的囊重抑制率、抗体及其亚类的变化。方法将细粒棘球绦虫重组Bb-Eg95-EgA31疫苗分别采用皮下注射、肌肉注射、鼻腔内接种和口服灌胃4种途径免疫BALB/c小鼠,免疫后8周每鼠用50个Eg原头节攻击感染,感染后25周剖杀小鼠,分离细粒棘球蚴包囊并称重,计算囊重抑制率;收集血清,常规ELISA测定血清IgG及其亚类和IgE水平,试验设有空载体、Bb和MRS对照。结果皮下注射组、肌肉注射组、鼻腔内接种组和口服灌胃组免疫小鼠的囊重抑制率分别为45.33%、41.33%、70.67%和62.67%,血清IgGI、gG2aI、gG2b和IgG1水平显著升高,IgG3和IgE水平显著降低。结论接种Bb-Eg95-EgA31疫苗能诱导小鼠产生保护力,IgGI、gG2aI、gG2b和IgG1起重要作用。
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| 关 键 词： | 细粒棘球绦虫 重组Bb-Eg95-EgA31疫苗 保护力 |
Protective immunity in mice elicited by a recombinant Bb-Eg95-EgA31 vaccine and challenged with protoscoleces of Echinococcus granulosus |
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| ZHOU Bi-ying,,CHEN Ya-tang,LI Wen-gui,YANG Mei. Protective immunity in mice elicited by a recombinant Bb-Eg95-EgA31 vaccine and challenged with protoscoleces of Echinococcus granulosus[J]. Journal of Pathogen Biology, 2010, 0(2) |
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| Authors: | ZHOU Bi-ying CHEN Ya-tang LI Wen-gui YANG Mei |
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| Affiliation: | ZHOU Bi-ying1,2,CHEN Ya-tang1,LI Wen-gui1,YANG Mei 1(1.Institute of Infectious , Parasitic Diseases,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China,2.Department of Parasitology,Zunyi Medical College,Zunyi 563003,Guizhou,China) |
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| Abstract: | Objective To investigate the reduction of hydatid cysts and the level of immunoglobulin(Ig) in mice immunized with a recombinant Bifidobacteria bifidum(Bb) Bb-Eg95-EgA31 vaccine of Echinococcus granulosus(Eg) and challenged with Eg protoscoleces.Methods BALB/c mice were respectively immunized with the vaccine subcutaneously,intramuscularly,intranasally,and orally.Mice were then challenged with 50 Eg protoscoleces 8 weeks after vaccination and sacrificed 25 weeks after infection.The weight of hydatid cysts w... |
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| Keywords: | Echinococcus granulosus recombinant Bb-Eg95-EgA31 vaccine protection |
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