| Aging-related Changes of Microglia and Astrocytes in Hypothalamus after Intraperitoneal Injection of Hypertonic Saline in Rats |
| |
| 作者姓名： | 王小丽 许耘 王芳 唐立华 刘子龙 李宏莲 刘胜洪 |
| |
| 作者单位： | Department of Histology and Embryology Huazhong University of Science and Technology Wuhan 430030，China，Department of Histology and Embryology，Huazhong University of Science and Technology Wuhan 430030，China，Department of Histology and Embryology，Huazhong University of Science and Technology Wuhan 430030，China，Department of Histology and Embryology，Huazhong University of Science and Technology Wuhan 430030，China，Institute of Reproductive Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan 430030，China，Department of Histology and Embryology，Huazhong University of Science and Technology Wuhan 430030，China，Department of Histology and Embryology，Huazhong University of Science and Technology Wuhan 430030，China |
| |
| 基金项目： | This project was supported by a grant from Jiang XueyiFundation of Tongji Medical College , Huazhong Universityof Science and Technology , China |
| |
| 摘 要： | Hypothalamus is a veryi mportant areain cen-tral nervous system, which takes part in the regu-lation of osmotic pressure . There are many kindsof neurons in this location that are involved in thesynthesis and the release of vasopressin and main-tain the balance of inner osmotic pressure by regu-lating the function of kidneys .It was reported thatmany neurons in hypothalamus showed aged-relat-ed change as a result of the alteration of osmoticpressure .But it remains unclear whether the neu-rogl…
|
| 关 键 词： | 老化 小神经胶质细胞 星形细胞 视丘下部 腹膜内压力 |
| 收稿时间： | 2005-12-01 |
Aging-related changes of microglia and astrocytes in hypothalamus after intraperitoneal injection of hypertonic saline in rats |
| |
| Wang Xiaoli,Xu Yun,Wang Fang,Tang Lihua,Liu Zhilong,Li Honglian,Liu Shenghong.Aging-related Changes of Microglia and Astrocytes in Hypothalamus after Intraperitoneal Injection of Hypertonic Saline in Rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(2):231-234. |
| |
| Authors: | Wang Xiaoli Xu Yun Wang Fang Tang Lihua Liu Zhilong Li Honglian Liu Shenghong |
| |
| Institution: | 1. Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Institute of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
| Abstract: | To examine the aging-related changes of microglia and astrocytes in hypothalamus of rats after intraperitoneal injection of hypertonic saline in rats, old- and young-aged rats were injected with hypertonic saline solution into peritoneal cavity. Lectin histochemical techniques using Ricinus communis agglutinin-1 (RCA-1) and immunocytochemical method employing antibody against glial fibrillary acidic protein (GFAP) were used to demonstrate microglia and astrocytes in the hypothalamus of the rats, and the positively-stained cells were analyzed by computer-assisted image analysis system. Our results showed that the numbers of microglia and astrocytes were significantly increased in the hypothalamus of old-aged rats. After intraperitoneal injection of hypertonic saline, the number of microglia was significantly decreased in the hypothalamus of both young- and old-aged groups. After introperitoneal injection of hypertonic saline, the number of GFAP positive cells was significantly increased in the hypothalamus of young rats, but the number of GFAP positive cells did not show significant change in the hypothalamus of old rats. It is concluded that in the hypothalamus of old-aged rats, the increase of microglia may be related with the aging or degeneration of neurons, and the increase of astrocytes may provide more nourishment required by the aged neurons. The microglia and astrocytes in the hypothalamus of the two group rats may be affected by hypertonic saline, and the response of these cells to the stimuli is characterized by some aging-related changes. |
| |
| Keywords: | microglia astrocyte aging hypertonic stimulation |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
