| 藻糖蛋白单糖组成两种分析方法的初步探讨 |
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| 引用本文： | 宋玉娟,陈亚飞,韩春霞,范慧红.藻糖蛋白单糖组成两种分析方法的初步探讨[J].中国生化药物杂志,2009,30(1). |
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| 作者姓名： | 宋玉娟 陈亚飞 韩春霞 范慧红 |
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| 作者单位： | 1. 中国药品生物制品检定所,北京,100050
2. 河南省食品药品检验所,河南,郑州,450003 |
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| 摘 要： | 目的探讨藻糖蛋白单糖组成的测定方法。方法采用气相色谱法和离子色谱法测定单糖组成。结果气相色谱法1不适于藻糖蛋白单糖分析,气相色谱法2较适合藻糖蛋白单糖分析;离子色谱法测得藻糖蛋白含7种单糖。结论两种方法均可用于单糖的定性定量分析,灵敏度高,重现性好。
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| 关 键 词： | 单糖 离子色谱 气相色谱 |
Initial study on two determination methods of monosaccharide in spirulina glucoprotein |
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| SONG Yu-juan,CHEN Ya-fei,HAN Chun-xia,FAN Hui-hong.Initial study on two determination methods of monosaccharide in spirulina glucoprotein[J].Chinese Journal of Biochemical Pharmaceutics,2009,30(1). |
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| Authors: | SONG Yu-juan CHEN Ya-fei HAN Chun-xia FAN Hui-hong |
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| Institution: | 1.National Institute for the Control of Pharmaceutical and Biological Products;Beijing 100050;China;2.Henan Institute for Food and Drug Control;Zhengzhou 450003;China |
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| Abstract: | Purpose To compare the determination methods of monosaccharide in spirulina glucoprotein.Methods Gas and ion chromatography was used.Results Method 1 of gas chromatography can′t be used for determination of monosaccharide in spirulina glucoprotein,while method 2 was suitable,and 7 monosaccharides were found in ion chromatography.Conclusion The two methods can both determine the quality and quantity of monosaccharide and both have high sensitivity and repeatability. |
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| Keywords: | monosaccharide ions chromatography gas chromatography |
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