血吸虫病患者血清中循环25kD表膜抗原的检测

采用细胞杂交瘤技术获得一株血吸虫成虫抗原的单克隆抗体SM 38-3。其靶抗原为成虫表膜,免疫球蛋白型为1gG 2a,能识别血吸虫分子量为25k D的表膜蛋白抗原表位。该单克隆抗体纯化后标记过氧化物酶,用于Dot-ELISA测定慢性血吸虫病人血清115份,阳性反应103份,阳性率为89.6％;测定急性病人血清89份,阳性反应85份,阳性率为95.5％;测定141份正常人血清,阳性反应2份,假阳性率为1.4％。与血吸虫肠相关抗原的检测比较,结果阳性率与抗原滴度均无明显差别。

THE DETECTION OF CIRCULATING 25kD TEGUMENT ASSOCIATED ANTIGEN IN SERA FROM SCHISTOSOMIASIS PATIENTS

The monoclonal antibody against schistosome adult worms was obtained by means of the hybridoma technique. Its target is of the tegument of adult worm and isotype is of IgG2a,recognising a epitope of 25kD tegument protein antigen. The monoclonal antibody was pu- rified and labelled with peroxidase, then was used in Dot -ELISA for detecting the circulating tegument associated antigen. Sera of 115 patients of chronic schistosomiasis parasitologically confirmed were tested, 103 were positive with a positive rate of 89. 6%. Sera of 89 patients of acute schistosomiasis were tested, 85 were positive with a positive rate of 95. 5%. Sera of 114 normal individuals were exam- ined,2 were positive with a false positive rate of 1. 4%. When the positive rate and antigen titer on circulating tegument associated antigen were compared with on the gut associated antigen, there was no obviously different.