HPV16 E6 DNA疫苗诱导小鼠抗宫颈癌主动免疫

目的：探讨HPV16 E6 DNA疫苗能否在体内诱导小鼠抗宫颈癌主动免疫应答。 方法：将HPV16 E6基因转染615小鼠宫颈癌U14，建立高表达E6的U14细胞株即E6+U14；制备HPV16 E6 DNA疫苗；随后分组进行体内预防和治疗E6+U14移植同系小鼠试验，设立对照组；观察各组小鼠肿瘤大小，计算抑瘤率、生存时间；计算淋巴和肺转移率；体外分别检测经HPV16 E6 DNA疫苗免疫后的小鼠T 淋巴细胞对E6+U14和U14的杀伤作用。结果：HPV16 E6 DNA疫苗预防和治疗组小鼠各时期瘤体大小均显著低于对照组（均P<0.001 ），两组抑瘤率均大于70%；平均生存时间均大于对照组（均P<0.001）；预防组淋巴转移率低于对照组（P<0.05）；HPV16 E6 DNA疫苗免疫小鼠T淋巴细胞在不同效靶比，对E6+U14的杀伤效率均明显高于对U14者（均P<0.001）。结论：HPV16 E6 DNA疫苗能诱导机体产生针对HPV16 E6阳性的宫颈癌细胞主动免疫应答及特异性细胞毒淋巴细胞，提示该疫苗可能对临床治疗宫颈癌有效。

Antitumor immune response to cervix carcinoma in mice induced by HPV16 E6 DNA vaccines

OBJECTIVE: To investigate the effect of HPV16E6 DNA vaccines on inducing the antitumor immune response to cervix carcinoma in vivo. METHODS: We prepared pCB/HPV16E6 DNA vaccines to prevent and treat 615 modle mice transplanted E6 + U14 in vivo respectively. Survival and tumor sizes of all mice were recorded and their lymphnodes and lung's metastases were detected by microscopic observation. The cytotoxicity of the spleen T cells of those mice immuned with HPV E6 DNA vaccines against the E6 U14 and U14 was detected by MTT assay. RESULTS: HPV16 E6 DNA vaccines prevented the transplanted tumor growth effectively in inbred strain mice and cured some tumor-bearing mice (P < 0. 001). Pathological results showed that the number of lymphodes with metastases foci decreased in the preventing group (P < 0.05). HPV16 E6 DNA vaccines induced special and higher cytotoxicity T lymphocytes against the E6 + U14 (P < 0.001). CONCLUSION: HPV16 E6 DNA vaccines can induce antitumor immune protection of the mice against E6 + U14 cells, suggesting that the vaccines may be effective in preventing and treating cervix cancer after an operation.