GC测定消炎止痒醑中樟脑的含量

目的建立GC测定消炎止痒醑中樟脑的含量。方法采用毛细管气相色谱法。色谱柱为CP-Sil 5 CB石英毛细管色谱柱(25 m×0.32 mm,0.4μm);FID火焰离子化检测器;载气为氮气;氢气与氮气的流量比通常为1∶1~1∶1.5;氢气和空气流量比为1∶1 0;流速:1 mL.min 1;分流比:20∶1;温度设置:进样口温度为250℃,柱温为280℃(程序升温:从60℃开始,保持1 min,再以12℃.min1的速度升温至280℃,保持3 min),检测器温度为300℃;进样量:1μL。结果樟脑在50~400 mg.L1内呈现良好的线性关系,r=0.999 9;平均回收率为99.64%(RSD为1.19%);在6 h内稳定。结论本方法简单易行、快速、准确性和重复性好,可用于消炎止痒醑的质量控制。

Determination of Camphor in Xiaoyanzhiyang Xu by GC

OBJECTIVE To establish a method for determine camphor in Xiaoyanzhiyang Xu by GC. METHODS Use capillary gas chromatography. Chromatographic column is CP-Sil 5 CB quartz chromatographic column（25 m × 0.32 mm, 0.4 μm）; FID flame ionization detector; carrier gas is nitrogen; usually specific flux of hydrogen and nitrogen is 1 ： 1-1 ： 1.5; flow ratio of hydrogen and air is 1 ： 10; velocity of flow： 1 mL.min-1; split ratio： 20 ： 1; temperature setting： sampling input temperature 250 ℃; column temperature 280℃（temperature programming process： start from 60℃, retain 1 min, achieve 280℃ by temperature rising speed 12 ℃.min-1, retain 3 min）; detector temperature 300 ℃; sample size： 1 μL. RESULTS The linear range of Camphor was 50-400 mg.L-1, r=0.999 9; the recovery of Camphor was 99.64%（RSD=1.19%） and it was stable in 6 hours. CONCLUSION The method is simple and easy, fast, accurate and repeatable. It can be used as quality control method for Xiaoyanzhiyang Xu.