HPLC测定微球中盐酸左氧氟沙星的含量

目的 建立测定壳聚糖微球中盐酸左氧氟沙星含量的高效液相色谱法。方法 采用Agilent 1200型高效液相色谱仪，色谱柱为ZORBAX Eclipse XDB-C₁₈(4.6 mm×150 mm，5 μm)，柱温：30 ℃，流速：1.0 mL·min^-1，流动相：0.04 mol·L^-1磷酸溶液(三乙胺调pH值为4)-乙腈(85∶15)，检测波长：293 nm。结果 盐酸左氧氟沙星浓度在1.021~51.15 μg·mL^-1内线性关系良好(r=0.999 6)，日内和日间精密度RSD均<2%(n=5)，平均加样回收率为99.1%，RSD为0.72%。结论 本方法简便易行、灵敏度高、重复性好，结果准确可靠，适用于壳聚糖微球中盐酸左氧氟沙星含量测定。

Determination of Levofloxacin Hydrochloride in Microspheres by HPLC

OBJECTIVE To establish an HPLC method for determination of levofloxacin hydrochloride in chitosan microspheres.METHODS The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm） at 30 ℃.The mobile phase was a mixture of 0.04 mol·L 1 phosphoric acid（adjusted to pH 4 with triethylamine） and acetonitrile（85∶15）.Flow rate was 1.0 mL·min 1 and UV detection wavelength was 293 nm.RESULTS Good liner relationship was obtained in the range of 1.021 51.15 μg·mL 1（r=0.999 6）.Both intra-and inter-day precision RSD were less than 2%（n=5）.The average recovery rate was 99.1% with RSD of 0.72%.CONCLUSIONS The established method is sensitive,reliable and suitable for quantitative analysis of levofloxacin hydrochloride in chitosan microspheres.