| 特异性抑制环氧合酶-2表达的siRNA筛选 |
| |
| 引用本文： | 沈雁,李瑞珺,范达,李谦,涂家生.特异性抑制环氧合酶-2表达的siRNA筛选[J].中国现代应用药学,2012,29(5):377-380. |
| |
| 作者姓名： | 沈雁 李瑞珺 范达 李谦 涂家生 |
| |
| 作者单位： | 中国药科大学药剂学教研室,中国药科大学药剂学教研室,中国药科大学药剂学教研室,中国药科大学生命科学院,中国药科大学药剂学教研室;中国药科大学生命科学院;南京 |
| |
| 基金项目： | 国家科技支撑计划(2008BAI55B03); 国家自然科学基金(81072588) |
| |
| 摘 要： | 目的筛选有效抑制环氧合酶-2(COX-2)表达的小干扰RNA(siRNA)序列,并观察其对细胞中COX-2表达的抑制作用。方法设计针对COX-2的3对(siRNA24,siRNA954,siRNA1553)及一条FAM荧光标记的阴性对照siRNA(称为HK)。在Lipofectamine2000介导下转染人胃癌细胞系SGC-7901。同时采用RT-PCR法和Western blot方法检测细胞中COX-2含量,以β-actin蛋白做内参照。结果当Lipofectamine2000浓度在50μmol.L 1时能实现最大转染效果。与其他各组相比,转染了siRNA954的人胃癌细胞中,编码COX-2 mRNA的含量明显减少(P<0.01)。结论成功设计了针对COX-2的siRNA,并从中筛选出siRNA954,能够有效抑制人胃癌细胞COX-2表达,为肿瘤治疗及其临床应用奠定了基础。
|
| 关 键 词： | 环氧合酶-2 siRNA RT-PCR 转染 |
| 收稿时间： | 9/6/2011 9:22:40 AM |
| 修稿时间： | 1/12/2012 1:08:31 PM |
Selection of Specific siRNA Inhibiting the Expression of COX-2 |
| |
| SHEN Yan,LI Ruijun,FAN D,LI Qian,TU Jiasheng.Selection of Specific siRNA Inhibiting the Expression of COX-2[J].The Chinese Journal of Modern Applied Pharmacy,2012,29(5):377-380. |
| |
| Authors: | SHEN Yan LI Ruijun FAN D LI Qian TU Jiasheng |
| |
| Institution: | Department of Pharmaceutics,China Pharmaceutical University,Department of Pharmaceutics,China Pharmaceutical University,Department of Pharmaceutics,China Pharmaceutical University,School of Life Science and Technology;China Pharmaceutical University;,Department of Pharmaceutics,China Pharmaceutical University; |
| |
| Abstract: | OBJECTIVE To select the siRNA which could most effectively inhibit the expression of COX-2,and to observe the change of COX-2 expression in cells.METHODS Three siRNAs were designed（siRNA24,siRNA954,siRNA1553） and one FAM fluorescence-labeled siRNA was used as a negative control（HK）.Detect the effect of inhibition by RT-PCR and Western blot after transfecting human gastric cancer cell line SGC-7901.RESULTS The 50 μmol·L 1 Lipofectamine2000 had highest transfection effiency.The expression of human COX-2 gene was suppressed specific and effectively by siRNA954,compared with other groups（P0.01）.CONCLUSION siRNA954 can inhibit COX-2 expression effectively.It plays an important role in the treatment of tumor. |
| |
| Keywords: | COX-2 siRNA RT-PCR transfection |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国现代应用药学》浏览原始摘要信息 |
| 点击此处可从《中国现代应用药学》下载免费的PDF全文 |
|
