肺癌组织端粒酶活性的定量研究及其临床意义

目的 研究肺癌组织中端粒酶活性的表达及其临床意义。方法 采用定量放射端粒重复序列扩增法（ＴＲＡＰ）检测７４例肺组织中（４５例为ＮＳＣＬＣ,１５例ＳＣＬＣ,结核７例,炎性假瘤７例）端粒酶的活性。结果 ＮＳＣＬＣ端粒酶阳性率为７３．３％,且活性较弱（１７２ＴＰＧ）,ＳＣＬＣ端粒酶的阳性率为９３．３％,且活性较强（２８８ＴＰＧ）。结核与炎性假瘤端粒酶 性全为阴性,ＳＣＬＣ端粒酶活性显著高于ＮＳＣＬＣ（Ｐ

A study on the telomerase activity in lung cancer and its clinical significance.

Objective To investigate the telomerase activity in human lung cancer and its clinical significance. Methods Sixty human lung cancer specimens (NSCLC 45, SCLC 15), seven tuberculosis specimens and seven inflammatory pesudotumor were examined for telomerase activity by means of TRAP (telomeric repeat amplification protocol) based on polymerase chain reaction. Results In NSCLC, telomerase positivity was 73.3%(33/45) with weak to moderate activity (172?TPG), whereas 14 of 15 SCLC (93.3%) were positive with strong activity (288?TPG), and all tuberculosis and inflammatory pseudotumor were telomerase negativity. The telomerase activity was significantly higher in SCLC than that in NSCLC (P<0.05), and was closely associated with the differentiation, size of the cancer, lymph node metastasis, and TNM staging (P<0.05). Conclusion The results suggest that telomerase may be regarded as one of the prognostic factor in lung cancer.