Bile acid stimulation of colonic adenylate cyclase and secretion in the rabbit |
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| Authors: | Dr. Dean R. Conley MD Martin J. Coyne MD George G. Bonorris Ane Chung PhD Leslie J. Schoenfield MD PhD |
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| Affiliation: | (1) Division of Gastroenterology, Cedars-Sinai Medical Center, 8720 Beverly Boulevard, 90048 Los Angeles, California;(2) Knoxville Gastroenterology Group, Assoc., Suite 100, Physicians Office Building, 1928 Alcoa Highway, 37920 Knoxville, Tennessee |
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| Abstract: | In vitro studies have suggested that the cyclic AMP system may be the mediator of bile acid-induced colonic secretion. The aims of our experiments were to quantify thein vivo effect of various doses of deoxycholic acid (DCA) on adenylate cyclase activity (AC) and net secretion in the rabbit colon. AC increased significantly (P<0.01) with each increasing concentration of DCA; at 8 mM the activity was 126±6 pmoles cAMP/mg protein/min, or 4 times the control. Phosphodiesterase activity (PDE) was affected and significantly decreased (P<0.01) only by 8 mM DCA. The volume of luminal fluid increased significantly (P<0.01) as a bicarbonate-rich solution with 4, 6, and 8 mM DCA in graded fashion. In conclusion, stimulation of a colonic mucosal cAMP system is strongly implicated as mediating DCA-induced colonic secretion.Supported in part by NIH grant AM 15631. |
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