穿孔膜片钳记录猪冠状动脉平滑肌细胞K+电流技术初探

常规全细胞膜片钳模式形成的同时，细胞内液与电极液交换造成细胞内物质丢失，此现象对小细胞的影响更为明显。穿孔膜片钳技术使电极与细胞胞质之间保持电学连续性，它使细胞内环境保持稳定，利于研究胞内信息传导机制．并可改善常规全细胞膜片钳时破膜所造成的封接稳定性的破坏，有望提高实验的成功率。本研究报道应用此技术在猪冠状动脉平滑肌细胞上记录的全细胞K^ 电流和由大电导钙激活钾通道BKCa所介导的自发瞬时外向电流ISTOCs。

K+ current of porcine coronary artery SMC recorded by perforated- patch clamp

When conventional whole-cell patch-clamp is formed, the exchange of the pipette solution and the cytoplasm of the cell result in the loss or alteration of cytoplasmic compounds, especially in small cells such as our research objects. Perforated-patch clamp provides electrical continuity between the pipette and the cytoplasm of the cell without the loss or alteration of enzymes or second messengers critical to the function of ion channel. It can keep cell internal environment stabilization , thus it's propitious to research the mechanism of signal transduction , and is able to improve the tranquilization of seal . This method is hopeful to enhance experiments' successful rate. This paper reported the whole-cell K~+ current and ISTOCs carried by BKCa channels of porcine coronary artery SMC recorded by perforated-patch clamp.