三氧矿合物复合纤维粘连蛋白盖髓的扫描电镜观察

目的:采用扫描电子显微镜技术观察三氧矿合物复合纤维粘连蛋白作为盖髓剂形成修复性牙本质桥的超微结构特点并对其疗效进行评价。 方法:以4只猫为实验对象,每只猫分为A、B、C、D 4个区,每区选取3颗实验牙,机械暴露牙髓后,分别用三氧矿合物、三氧矿合物复合纤维粘连蛋白、氢氧化钙、纤维粘连蛋白与医用淀粉盖髓,盖髓12周后应用扫描电镜观察新形成的牙本质桥。 结果: 氢氧化钙组、三氧矿合物组可见不规则的牙本质桥结构。三氧矿合物复合纤维粘连蛋白组可见完整的牙本质桥。在纤维粘连蛋白和医用淀粉组,未见牙本质桥形成。结论: 三氧矿合物复合纤维粘连蛋白能刺激修复性牙本质形成,形成的牙本质桥完整,值得临床推广应用。

The scanning electronic microscope observation of mineral trioxide aggregate combined with fibronectin as pulp capping agent

AIM: To observe the effect of mineral trioxide aggregate (MTA) combined with fibronectin as pulp capping agents. METHODS: The 48 pulps of 4 cats were exposed mechanically and then capped directly with MTA, MTA combined with fibronectin, calcium hydroxide, fibronectin and starch as control. After 12 weeks observation, the experimental teeth were extracted and observed with scanning electronic microscope. RESULTS: In calcium hydroxide group and MTA group, specimens showed irregular dentin bridge structures along the pulp-MTA interface. In MTA combined with fibronectin group, continuous and complete dentin bridge structures were found along the pulp-MTA interface, the crystalline-like structures were in direct contact with the dentin wall of pulp chamber, the exposed pulp sites were closed by the dentin bridge. In fibronectin and starch group, no reparative dentin was observed. CONCLUSION: The present experiment indicated that MTA combined with fibronectin was an effective pulp-capping material and may have potential for clinical application.