精子动态与形态学关系及其临床应用价值

目的探讨精子动态与形态学的关系及其临床应用价值。方法采用计算机辅助精子分析(CASA)系统对290例男性不育患者进行精液常规分析,同时用Diff-Quik快速染色方法对精子进行形态染色,采用严格标准进行精子形态分类,计数正常及异常形态精子,并计算正常形态精子的百分率。结果精子活力正常者(a级≥25%或a+b级≥50%为标准)108例,占37.2%,精子正常形态率(43±14)%。精子活力低下者182例,占62.8%,精子正常形态率(19.2±0.9)%。2组相比差异有统计学意义(P<0.01)。精子形态正常组195例,占67.2%,异常组95例,占32.8%。精子形态正常组与异常组在曲线速度(VCL)、直线速度(VSL)、平均移动角度(MAD)、直线性(LIN)、前向性(STR)差别显著。结论精子动态和形态异常相伴产生,精液常规与精子形态学分析相结合,有助于提高男性不育症的诊断率及为不孕患者选择合适的助孕方式。

Relationship between sperm motility parameters and sperm morphology

Objective To evaluate the relationship between sperm motility parameters and sperm morphology and its clinical value.Methods The sperm kinesis parameters of 290 cases were analyzed by the WLJY-9000 color semen analysis system;the morphology of sperm was detected by using Diff-Quik staining assay,the sperm were classified according to strict criteria and the percentage of normal sperm morphology were calculated.Results There were 108 cases of normal sperm motility(a grade≥25% or a+b grade≥50% as standard),accounting for 37.2%,and normal sperm morphology rate was(42.7±13.6)%.There were 182 cases of poor sperm motility,accounting for 62.8%,and normal sperm morphology rate was(19.2±0.9)%,there was a significant difference compared between these two groups(P<0.01).There were 195 cases of normal sperm morphology,accounting for 67.2%,and 95 cases of abnormal sperm morphology,accounting for 32.8%.The VCL,VSL,MAD,LIN,STR in normal sperm morphology group were different with that in abnormal morphology group.Conclusion Poor sperm motility was always accompanied by abnormal sperm morphology,combination of semen and sperm morphological analysis will contribute to improve the diagnostic rate of male infertility and to choose the right assisted reproductive methods for infertility patients.