红景天苷壳聚糖纳米粒的制备及其体外释放性能研究

目的 以壳聚糖为载体制备红景天苷壳聚糖纳米粒(SA-CS-NPs),并考察其体外释药特性.方法 采用溶剂扩散-离子交联法制备SA-CS-NPs,考察其粒径分布和形态,并对SA-CS-NPs的包封率、载药量及其体外释药特性进行研究.结果 所制得的SA-CS-NPs呈球形或类球形,平均粒径为(247.5±23.8) nm(n=3),Zeta电位为(23.4±2.7) mV(n=3),多分散指数(PDI)为0.265±0.071(n=3)；平均包封率为(70.15±1.60)％,平均载药量为(14.03±0.32)％(n=3)；24h累积释放率达85％以上.结论 溶剂扩散-离子交联法制备SA-CS-NPs具有合适的粒径和包封率,并能达到缓释效果.

Preparation and in vitro drug release properties of salidroside-chitosan nanoparticles

Objective To prepare salidroside-chitosan nanoparticles (SA-CS-NPs) and to evaluate the properties of in vitro drug release. Methods SA-CS-NPs were firstly prepared by solvent diffusion-ionic crosslinking method. The particle size and polydispersity of SA-CS-NPs were determined and the morphology of nanoparticles was evaluated. The properties of encapsulation efficiency (EE), load efficiency (LE), and in vitro release of SA-CS-NPs were also evaluated using UPLC method. Results The nanoparticles were successfully prepared with the spherical shape or para-spherical shape. The mean particle size was (247.5 ± 23.8) nm with the polydispersity index (PDI) of 0.265 ± 0.071, and the Zeta potential was (23.4 ± 2.7) mV (n = 3). The EE was (70.15 ± 1.60)% and the LE was (14.03 ± 0.32)% (n = 3). The cumulative release rate of SA-CS-NPs within 24 h was over 85%. Conclusion SA-CS-NPs prepared by solvent diffusion-ionic crosslinking method show appropriate particle size and EE, and could exhibit sustained release properties in vitro.