肾衰养真胶囊对5/6肾切慢性肾衰竭营养不良大鼠下丘脑信号传导蛋白SOCS-3表达的影响

目的探讨细胞因子信号传导抑制剂-3(SOCS-3)在慢性肾衰竭营养不良大鼠中的表达情况以及肾衰养真胶囊的调节作用。方法5/6肾切除制作慢性肾衰竭大鼠模型,同时予4%酪蛋白饮食,检测血尿素氮、肌酐、白蛋白、血红蛋白等生化指标和体重,观察其营养不良发生时间,符合条件的随机分为模型组、肾衰养真胶囊治疗组和开同治疗组;另设正常对照组。灌胃4周后应用放射免疫法检测血瘦素水平,免疫组织化学方法检测下丘脑SOCS-3蛋白的表达。结果慢性肾衰竭大鼠在术后10周末出现营养不良,肾衰养真胶囊可减低血浆尿素氮、肌酐、瘦素水平,增加白蛋白、血红蛋白水平;与正常组比较,模型组与肾衰养真胶囊治疗组SOCS-3当色阳性信号均强(P<0.001),但肾衰养真胶囊组SOCS-3染色阳性信号显著高于模型组(P<0.05)。结论肾衰养真胶囊可能部分是通过上调抑制蛋白SOCS-3,降低瘦素水平,从而改善慢性肾衰竭大鼠营养不良。

Effects of Shenshuai Yangzhen Capsule on SOCS-3 in Hapotholums in Malnutrition Rats with Chronic Renal Failure by 5/6 Nephrectomy

Objective To investigate the effects of Shenshuai Yangzhen capsule on SOCS-3 in hypothalamus in malnutrition rats with chronic renal failure by 5/6 nephrectomy.Methods SD rats were received 5/6 nephrectomy for preparation of CRF models,and fed with 4% casein at the same time.The blood parameters,like BUN,SCr,Alb,Hb and weight in CRF rats were determined.We observed when malnutrition began.Those in condition were randomized into CRF control group,ketosteric group,Shenshuai Yangzhen group,and normal control group.After 4-week treatment as indicated,the blood leptin in CRF rats were determined by radioimmunoassay.The protein expressions of SOCS-3 were assessed respectively by immunohestchemistry.Results Compared with those in the CRF group,the plasma blood urea nitrogen and serum creatinine concentrations and leptin contents in Shenshuai Yangzhen group were significantly lower with substantially elevated plasma album and haemoglobin.Compared with the normal group rats,the respective expression of SOCS-3 significantly increased in rats with surgically induced renal failure(two-stage subetotal nephrectomy)(P<0.001).After treatment with Shenshuai Yangzhen capsule,the expression of SOCS-3 in the CRF rats was upregulated(all P<0.05).Conclusion SOCS-3 proteins may be involved in the malnutrition associated with CRF.Upregulation of SOCS-3 may be responsible for the malnutrition effects of Shenshuai Yangzhen capsule in CRF rats.