复方胡黄连对D-半乳糖致急性肝损伤的保护作用及机制

目的 探讨复方胡黄连对D-半乳糖致急性肝损伤的保护作用及机制.方法 昆明种小鼠60只,随机分为空白对照组,模型对照组,阳性对照药肝加欣组(545 mg/kg),复方胡黄连高(34 mg/kg)、中(17 mg/kg)、低(8.5 mg/kg)剂量组,每组10只,连续灌胃7 d,于第7天早6:00灌胃给药,2 h后造模,除空白对照组外,其余各组腹腔注射0.1%D-半乳糖,体积为0.3 mL/10 g,24 h后摘眼球取血,用全自动生化分析仪测定ALT、AST,取出肝脏组织,根据试剂盒的要求测定SOD、MDA的含量.结果 复方胡黄连对D-半乳糖致小鼠急性肝损伤的治疗,模型组与空白组比较,其血清ALT和AST均明显升高(P<0.01),肝组织中SOD显著降低,MDA含量显著升高.复方胡黄连各给药组与模型组比较,均能显著降低血清ALT和AST含量(P<0.01),升高肝组织中SOD活性,降低MDA含量(P<0.01),改善其损伤的病理结构.结论 复方胡黄连对D-半乳糖致小鼠急性肝损伤的治疗,能显著降低血清ALT、AST活性,升高肝组织中SOD含量,降低MDA含量.

Protective effect and mechanism of compound Figwortflower Picrorhiza Rhizome on acute liver injury induced by D-galactose

Objective To explore the protective effect and mechanism of compound Figwortflower Picrorhiza Rhizome on the acute liver injury induced by D-galactose. Methods Sixty Kunming rats were randomly divided into blank control group, model control group, positive control group (545 mg/kg) of liver with jiaxin tablets, compound Figwortflower Picrorhiza Rhizome high dose group (34 mg/kg), compound Figwortflower Picrorhiza Rhizome medium dose group (17 mg/kg), compound Figwortflower Picrorhiza Rhizome low dose group (8.5 mg/kg). There were 10 rats in each group, consecutive lavage for seven days. Give the tablets at 6 a.m. on the seventh day and mold in 2 hours. In addition to the blank group, the other groups were injected with 0.1% D-galactose, 0.3 mL/ 10g, and the blood was taken after 24 hours, with automatic biochemical analyzer determination of ALT, AST, remove the liver tissue, according to the requirement of the kit for determining the content of SOD, MDA. Results Compared with blank group, the serum levels of ALT and AST in model group were significantly increased (P < 0.01), SOD in the liver tissue decreased significantly, the MDA content increased significantly. Compound Figwortflower Picrorhiza Rhizome each dosage group compared with model group, can significantly reduce serum ALT and AST levels (P< 0.01), increase SOD activity in liver tissue, reduce MDA content (P < 0.01), pathological structure to improve its damage. Conclusion The treatment of acute liver injury induced by compound Figwortflower Picrorhiza Rhizome to rats with D-galactose, which can significantly reduce the serum ALT and AST activity, can significantly increase SOD content in liver tissues and decrease MDA content.