新老产地川芎中3种内酯成分的含量测定

目的: 建立同时测定川芎中主要内酯成分(洋川芎内酯A、阿魏酸松柏酯、藁苯内酯)的方法,比较新老产地川芎药材中3种内酯成分的含量差异。 方法: 采用单因素考察同时提取3种内酯成分的最佳条件,采用反相高效液相色谱法测定3种内酯成分含量。 结果: 新老产地川芎中3种内酯成分含量差异较大,其中差异最大为阿魏酸松柏酯,其次是洋川芎内酯A、藁苯内酯。通过聚类分析发现3种内酯成分没有地域分布规律。 结论: 所建立的方法操作简便、灵敏快速、重复性好,可用于川芎药材的质量控制;新老产地川芎的内酯含量比较为川芎的引种栽培、品质评价供依据。

Determination and Comparison of the Three Lactones in Rhizoma Ligusticum chuanxiong from New and Traditional Producing Areas

Objective: To establish the method of determining the main lactones (senkyunolide A, coniferyl ferulate and ligustilide) in Ligusticum chuanxiong simultaneously, and to compare the content of them in L.chuanxiong from new and traditional producing areas. Method: The optimum condition of extracting three lactones was confirmed by single factor experiments. The content of the three was determined by RP-HPLC. Result: The content of the three lactones had significant differences between new and traditional producing areas, of which coniferyl ferulate was the most different, then senkyunolide A and ligustilide. Clustering analysis showed that there were no geographical distribution laws of the three lactones. Conclusion: The method was simple for operation, sensitive with good reproducibility and can be used for quality control of L.chuanxiong. The comparison of the three lactones from new and traditional producing areas can provide theoretical basis for introduction cultivation and quality evaluation of L.chuanxiong.