醋制老瓜头镇痛抗炎活性研究

目的: 研究醋制老瓜头二氯甲烷、乙酸乙酯、正丁醇层萃取物抗炎、镇痛活性,明确活性部位。 方法: 将小鼠分为11组,分别为:阳性对照组、模型组、醋制老瓜头二氯甲烷萃取物,乙酸乙酯萃取物,正丁醇萃取物分别设低、中、高剂量组(按生药量计为400,800,1 600 mg·kg^-1),给药3 d后进行实验,以热板法、醋酸扭体法为镇痛实验模型,二甲苯致小鼠耳肿胀、角叉菜胶致小鼠足肿胀、小鼠炎性组织前列腺素E₂(PGE₂)含量为抗炎实验模型,进行活性测定。 结果: 镇痛实验,热板法模型中醋制老瓜头各萃取层与模型组比较能显著延长热板所致的小鼠舔后肢的时间,扭体法模型中,醋制老瓜头各萃取层能显著减少醋酸致小鼠扭体反应的次数,并呈现一定的药物依赖性(P<0.05,P<0.01);抗炎实验,二甲苯致耳肿胀模型中醋制老瓜头各萃取层与模型组比较能不同程度地抑制二甲苯致小鼠耳肿胀,角叉菜胶致鼠足肿胀模型中醋制老瓜头各萃取层能不同程度抑制角叉菜胶致鼠足肿胀,各萃取层能显著降低小鼠炎症组织中PGE₂含量(P<0.05,P<0.01)。 结论: 二氯甲烷层为醋制老瓜头镇痛、抗炎活性最强部位,醋制降毒后仍有较强生物活性,表现为延长小鼠舔后肢的时间,抑制二甲苯致小鼠耳肿胀、角叉菜胶致足肿胀并显著降低小鼠炎症组织中PGE₂含量,其抗炎作用可能与PGE₂相关。

Study On Anti-inflammatory and Analgesic Effects of Different Extracts from Vinegar-processed Cynanchum komarovii

Objective: To observe the anti-inflammatory and analgesic effects of dichloromethane, acetate, and n-butanol extracts from vinegar-processed Cynanchum komarovii and to ensure the active fractions. Method: The mice were divided into 11 groups respectively. The effects were investigated 3 days after drug administration.Hot plate and glacial acetic acid writhing test were adopted in Kunming mice to observe the analgesic effect and the experiment of mice ear swelling induced by xylene and paw edema induced by carrageenan were applied to study on the anti-inflammatory effect. Result: Compared with the model group, all the three extracts of dichloromethane, acetate, and n-butanol (400, 800, 1 600 mg·kg^-1) from vinegar-processed C. komarovii could significantly prolong the time period of paw-licking and reduced the writhing times induced by glacial acetic acid in a dose-dependence (P<0.05, P<0.01). And then the different extracts could inhibit the ear swelling and paw edema, and decrease the content of prostaglandin E₂(PGE₂) in inflammation tissue. Conclusion: Dichloromethane extract was ensured to be the active fraction. After toxicity attenuation by vinegar-processing, the C. komarovii still has a stronger bioactivity that can prolong the time period of paw-licking and reduced the writhing times induced by glacial acetic acid and inhibit the ear swelling and paw edema, and decrease the content of PGE₂ in inflammation tissue. And the anti-inflammatory effect showed a relationship with content of PGE₂.