人参提取物对C57小鼠生发作用的影响

目的: 研究人参提取物对C57BL/6J小鼠毛发生长促进作用及其机制。 方法: 实验分为阳性对照组(章光101)、空白对照组、人参提取物高、中、低(2, 1, 0.5 g·mL^-1)剂量组。小鼠脱毛,通过观察脱毛区肤色的变化和组织学检查,研究人参提取物对小鼠毛发生长周期的影响;采用红外测温仪观察对小鼠皮肤温度的影响;采用浊度法和底物发色法,观察其抗血小板聚集的作用,初步探讨其促进毛发生长的机制。 结果: 人参各剂量组均能诱导C57BL/6J小鼠毛发生长,使其从休止期进入生长期,但对毛发的最终长度无影响,且呈现一定的剂量依赖作用(P<0.01),高剂量组与阳性组无显著性差异;组织学检查显示人参提取物可以刺激毛囊生长,产生黑色素;给药20,60 min后,人参各剂量组均能显著提高小鼠的体温(P<0.01),120 min后,恢复到给药前水平;人参高、中、低剂量组对血小板活化因子(PAF)诱导的大鼠5 min时血小板聚集率分别为49.41%,27.80%,18.39%(均P<0.01),且呈现一定的剂量依赖作用,与阳性对照组则无显著性差异;其抗血小板聚集的作用与抗凝血酶有关, 人参高、中、低剂量组的凝血酶抑制率分别为(20.24±2.72)%,(15.42±1.03)%,(8.61±0.80)%(P<0.01)。 结论: 人参提取物具有明显的促进毛发生长的作用,其机制与扩张皮肤毛细血管、改善局部微循环有关。

Experimental Study on the Effects of the Extract of Ginseng Radix et Rhizoma on Hair Growth of C57BL/6J Mice

Objective: To study the effects and mechanism of the extract of Ginseng Radix et Rhizoma on hair growth of C57BL/6J mice. Method: This experiment had 5 groups: positive control group (zhangguang 101), blank control group, high dose group (2 g·mL^-1), medium dose group (1 g·mL^-1), low dose group (0.5 g·mL^-1). The skin color and histological examination of hairless mice were investigated to study the effects of promoting hair growth. The skin temperature at different time was measured with infrared radiation thermometers and platelet aggregation rate in vitro was surveyed with nephelometry and chromogenic substrate to preliminarily study the mechanism. Result: All dose groups could induce the hair growth of C57BL/6J mice in a dose-dependent manner from resting stage to growing period, but had no effect on final length of the hair. The differences were significant (P<0.01) between the therapeutic groups and the controls, but no significant between high dose group and positive control group. Histologic examination showed that the extract of Radix Ginseng could produce melanin and induce C57BL/6 mice hair follicle to enter from telogen into anagen. Twenty and sixty minutes after administration, all dose groups can significantly improve the temperature of mice (P<0.01), however, one hundred and twenty minutes later, the temperature recovered to initial time. The blood platelet aggregation induced by platelet activating factor (PAF) at five minutes could significantly and concentration-dependently be inhibited by all dose groups, the platelet aggregation rates of different doses were 49.41%, 27.80%, 18.39%, repevtively, and the differences were significant between the therapeutic groups and the blank control group (P<0.01), but there were no significant differences between the Ginseng Radix et Rhizoma groups and the positive control group. The platelet aggregation activity was partially related to antithrombin and the thrombin inhibition rates of different doses were (20.24±2.72)%, (15.42±1.03)%, (8.61±0.80)%, respectively. The differences were significant between the therapeutic groups and the blank control group (P<0.01). Conclusion: The mechanism of the extract of Ginseng Radix et Rhizoma on hair growth of C57BL/6J could be connected with expending skin capillary and improving local microcirculatory perfusion.