不同生长年限苦参不同部位的生物碱含量

目的: 对不同年限苦参药材茎、叶、芦头、侧根、根5个部分总生物碱与5种单体生物碱进行含量测定。 方法: 采用高效液相色谱法分析不同年限苦参药材不同部位中5种指标性生物碱的含量。 结果: 5种指标性生物碱在不同生长年限 苦参不同部位的分布有不同特点,不同部位槐果碱的含量分布叶>茎>侧根>主根>芦头,苦参碱的分布是叶>茎>芦头>侧根>主根,氧化槐果碱的分布是侧根>主根>芦头>茎>叶,槐定碱的分布是侧根>主根>叶>茎>芦头,氧化苦参碱的分布是侧根>主根>芦头>茎>叶。随着生长年限的增加,各指标性生物碱成分含量随之增加,第4年有效成分增加幅度较小,其中主根中5种指标性成分含量依次为一年生13.58 mg·g^-1,两年生20.49 mg·g^-1,三年生27.74 mg·g^-1,四年生31.32 mg·g^-1。 结论: 上述结果为合理开发利用苦参以及苦参的生长年限提供了实验依据。

Determination of Five Alkaloids from Sophora flavescens of Different Parts and Different Growth Years

Objective: To determine the five alkaloids of different parts in S. flavescens of different growth years. Method: The five alkaloids in S. flavescens were analyzed by HPLC. Result: Five alkaloids of different parts in S. flavescens of different growth years had their distribution law. The content of sophocarpine's distribution in different parts was leaf>stem>lateral root>taproot>reed head; the distribution of matrine was leaf>stem>reed head>lateral root>taproot; the distribution of oxysophocarpine was lateral root>taproot>reed head>stem>leaf; the distribution of sophoridine was lateral root>taproot>leaf>stem>reed head; the distribution of oxymatrine was lateral root>taproot>reed head>stem>leaf. With the increase of growth years, the five alkaloids increased; the fourth year was tend to be gentle. For example, the content of the taproot was: 13.58 mg·g^-1 (first year), 20.49 mg·g^-1 (second year), 27.74 mg·g^-1(third year), 31.32 mg·g^-1(forth year). Conclusion: This research can provide data for rational utilization and growth years determination of S. flavescens.