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| 化橘红rDNA ITS序列特征的初步分析 | |
| 引用本文： | 陈晓颖,高晓霞,罗源生,蔡岳文,吴苑燕.化橘红rDNA ITS序列特征的初步分析[J].中药材,2007,30(3):268-270. |
| 作者姓名： | 陈晓颖 高晓霞 罗源生 蔡岳文 吴苑燕 |
| 作者单位： | 1. 广东药学院,广东广州,510006 2. 广东广弘药材有限公司,广东广州,510080 3. 广东省中药研究所,广东广州,510520 |
| 摘 要： | 目的:研究化橘红及其近缘种rDNA ITS区碱基序列的特征及差异。方法:利用PCR产物直接测序,并作序列变异分析。结果:5个植物样品的rDNA ITS序列长度为675bp-683bp,其中5.8S rDNA长度为165bp,编码区较保守。基于K2P参数遗传距离模型构建的化橘红与沙田柚、柑橘rDNA ITS序列遗传距离分别为1.05和2.29。结论:化橘红与其近缘品种rDNA ITS序列有明显的差异。 |
| 关 键 词： | 化橘红 沙田柚 rDNA ITS序列 |
| 文章编号： | 1001-4454(2007)03-0268-03 |
| 修稿时间： | 2006年7月12日 |
Preliminary Study on Rdna ITS Sequencing and Characteristics of Citrus grandis var. Tomentosa |
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| CHEN Xiao-ying,GAO Xiao-xia,LUO Yuan-sheng,CAI Yue-wen,WU Yuan-yan.Preliminary Study on Rdna ITS Sequencing and Characteristics of Citrus grandis var. Tomentosa[J].Jorunal of Chinese Medicinal Materials,2007,30(3):268-270. | |
| Authors: | CHEN Xiao-ying GAO Xiao-xia LUO Yuan-sheng CAI Yue-wen WU Yuan-yan |
| Institution: | Guangdong University of Pharmacy, Guangzhou 510006, China. |
| Abstract: | Objective: To study the difference of rDNA ITS sequences between Cirtus grandis var.tomentosa and its relatives.Methods: The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.Results: The DNA sequences of ITS ranged from 675 to 683 bp and the 5.8S gene of all of the samples are 165 bp in size.Distance values of ITS sequence,estimated according to Kimura two-parameter models between C.grandis var.tomentosa and C.grandis var.shatin-you,C.grandis var.tomentosa and C.reticulata were 1.05 and 2.29 respectively.Conclusion: There are some differences in rDNA ITS sequences among C.grandis var.tomentosa and its relatives. |
| Keywords: | Citrus grandis var tomentosa Hort Citrus grandis var shatin-you Hort rDNA internal transcribed spacers(ITS) sequence |
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