| 泛耐铜绿假单胞菌氨基糖苷类药物产酶耐药机制的研究 |
| |
| 引用本文： | 邓丹菲,胡锡浩,许小敏,冯伟云. 泛耐铜绿假单胞菌氨基糖苷类药物产酶耐药机制的研究[J]. 中国卫生检验杂志, 2010, 0(4) |
| |
| 作者姓名： | 邓丹菲 胡锡浩 许小敏 冯伟云 |
| |
| 作者单位： | 浙江省宁波市第二医院; |
| |
| 基金项目： | 宁波市自然科学基金(2008A610090) |
| |
| 摘 要： | 目的:了解我院临床分离泛耐铜绿假单胞菌(PDRPA)16S rRNA甲基化酶基因、氨基糖苷类药物修饰酶基因存在状况,探讨PDRPA氨基糖苷类耐药机制。方法:GNS-448药敏卡及K-B法测定抗菌药物的敏感性,聚合酶链反应(PCR)检测氨基糖苷类修饰酶基因、16S rRNA甲基化酶基因。结果:22株PDRPA对20种常用抗菌药物耐药,16S rRNA甲基化酶rmtB阳性1株(4.5%);氨基糖苷类修饰酶aac(6′)-Ⅰb阳性4株(18.2%),ant(3″)-Ⅰ阳性12株(54.5%),ant(2″)-Ⅰ阳性8株(36.4%),其他基因均阴性。共18株检出氨基糖苷类修饰酶基因。结论:PDRPA氨基糖苷类耐药为多重耐药机制,我院PDRPA氨基糖苷类耐药机制主要与氨基糖苷类修饰酶基因相关。
|
| 关 键 词： | 铜绿假单胞菌 氨基糖苷类修饰酶基因 16S rRNA甲基化酶基因 PCR |
Study on mechanisms of aminoglycoside enzyme-producing and drug resistance in pan-drug resistant Pseudomonas aeruginosa |
| |
| DENG Dan-fei,HU Xi-hao,XU Xiao-min,FENG Wei-yun. Study on mechanisms of aminoglycoside enzyme-producing and drug resistance in pan-drug resistant Pseudomonas aeruginosa[J]. Chinses Journal of Health Laboratory Technology, 2010, 0(4) |
| |
| Authors: | DENG Dan-fei HU Xi-hao XU Xiao-min FENG Wei-yun |
| |
| Affiliation: | DENG Dan-fei,HU Xi-hao,XU Xiao-min,FENG Wei-yun(Ningbo Second Hospital,Ningbo 315010,China) |
| |
| Abstract: | Objective:To investigate the status of the 16SrRNA methylases gene and aminoglycoside-modifying en-zyme gene in pan-drug resistant Pseudomonas aeruginosa(PDRPA) isolated from clinic,and further understand the resistant mechanisms of aminoglycoside drug.Methods:GNS-448 and K-B test was performed to detect the susceptibility of 19 kinds of antimicrobial agents against 22 strains of PDRPA.16SrRNA methylase genes and aminoglycoside-modifying enzyme genes were detected by polymerase chain reaction(PCR) and verif... |
| |
| Keywords: | Pseudomonas aeruginosa Aminoglycoside-modifying en-zyme gene 16S rRNA methylases gene PCR |
| 本文献已被 CNKI 等数据库收录！ |
|
