| 日本血吸虫14—3—3抗原编码基因的克隆及序列测定 |
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| 引用本文： | 汪学龙 沈继龙 等. 日本血吸虫14—3—3抗原编码基因的克隆及序列测定[J]. 中国人兽共患病杂志, 2001, 17(5): 45-47 |
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| 作者姓名： | 汪学龙 沈继龙 等 |
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| 作者单位： | 汪学龙（安徽医科大学病原生物学教研室，合肥 230032） 沈继龙（安徽医科大学病原生物学教研室，合肥 230032） 蒋作君（安徽医科大学病原生物学教研室，合肥 230032） |
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| 基金项目： | 国家自然科学基金(编号 39570646)和安徽省自然科学基金资助课题( 编号 97410042) |
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| 摘 要： | 为了寻找日本吸虫病新的诊断和候选疫苗分子，设计合成引物，以日本血吸虫成虫cDNA第一链为模板，用PCR法扩增出日本血吸虫14－3－3抗原（Sj14-3-3）基因编码序列，其大小约784bp，将其克隆入pGEM-T载体，重组质粒pGEM-T-Sj14-3-3经双酶切和以质粒为模板进行PCR扩增，均可获得一条与PCR产物一致的DNA片段，序列测定结果表明具有一个长度为765bp的完整开放阅读框，与曼氏血吸虫14－3－3核苷酸序列有高度同源性。本实验克隆了日本血吸虫14－3－3抗原的编码基因，并进行了序列测定，为进一步研究提供了条件。
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| 关 键 词： | 日本血吸虫 14－3－3抗原 基因克隆 序列测定 |
| 文章编号： | 1002-2694(2001)05-0045-03 |
| 修稿时间： | 2000-03-16 |
CLONING AND SEQUENCE OF 14-3-3 PROTEIN GENE OF SCHISTOSOMA JAPONICUM |
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| WANG Xuelong,SHEN Jilong,JIANG Zuojun. CLONING AND SEQUENCE OF 14-3-3 PROTEIN GENE OF SCHISTOSOMA JAPONICUM[J]. Chinese Journal of Zoonoses, 2001, 17(5): 45-47 |
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| Authors: | WANG Xuelong SHEN Jilong JIANG Zuojun |
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| Abstract: | In order to discover new candidate vaccine molecular for schistosomiasis,primers were designed and synthesized The DNA encoding 14-3-3 antigen of S japonicum was amplified by polymerase chain reaction using SjcDNA as template The product from PCR was cloned into pGEM-T vector and sequenced The recombinant vector (pGEM-Sj14-3-3) was identified by restriction analysis and PCR For PCR, a specific band of around 784bp was amplified The same band was obtained by double restriction of recombinant plasmids and PCR using recombinant plasmids as template The nucleotide sequence has an open reading frame of 765bp It provided the basis for further study on Sj14-3-3 expression and its immunological diagnosis and prevention |
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| Keywords: | Schistosoma japonicum 14-3-3 antigen Gene cloning Sequencing |
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